泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)多克隆抗體
Polyclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)
CI-75Kd; NADH-Coenzyme Q Reductase; NADH Dehydrogenase Iron-Sulfur Protein 1; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
- 編號PAJ794Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPJ794Hu01-泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30μg/mL Rabbit Anti-Human NDUFS1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Human Cerebrum Tissue; <br/>Primary Ab: 30μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Human Cardiac Muscle Tissue; <br/>Primary Ab: 20μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Samples: Human Kidney Tissue; <br/>Primary Ab: 20μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Human Cardiac Muscle Tissue<br/>Primary Ab: 20μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Human Kidney Tissue<br/>Primary Ab: 20μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
DAB staining on IHC-P;<br/>Sample: Human Cerebrum Tissue<br/>Primary Ab: 30μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Control: Used PBS instead of primary antibody<br/>Second Ab: 2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant NDUFS1, Human.
Knockout Varification: <br/>Lane 1: Wild-type A549 cell lysate; <br/>Lane 2: NDUFS1 knockout A549 cell lysate; <br/>Predicted MW: 81,79,74,68kd <br/>Observed MW: 74kd<br/>Primary Ab: 2μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human A549 cell lysate; <br/>Primary Ab: 2μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate; <br/>Primary Ab: 2μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Kidney lysate; <br/>Primary Ab: 2μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate; <br/>Primary Ab: 2μg/mL Rabbit Anti-Human NDUFS1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對NDUFS1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NDUFS1。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-30μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ794Hu01 | 泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ794Hu01 | 泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)多克隆抗體 | WB,IHC |
| MAJ794Hu21 | 泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEJ794Hu | 泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ794Hu | 泛醌NADH脫氫酶Fe-S蛋白1(NDUFS1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Brown and beige adipose tissue regulate systemic metabolism to resist diet-induced obesity through metabolite signals in an inter-organ signaling axis[] | |
| Nature Communications | Brown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis[33772024] |