親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Cyclophilin A (CYPA)
PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein
- 編號KSA979Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.55ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對親環(huán)素A(CYPA)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA979Mu01 | 親環(huán)素A(CYPA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA979Mu02 | 親環(huán)素A(CYPA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA979Mu02 | 親環(huán)素A(CYPA)多克隆抗體 | WB |
| PAA979Mu01 | 親環(huán)素A(CYPA)多克隆抗體 | WB; IHC |
| FAA979Mu01 | 抗親環(huán)素A(CYPA)多克隆抗體 | FCM |
| LAA979Mu81 | 親環(huán)素A(CYPA)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA979Mu71 | 親環(huán)素A(CYPA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA979Mu24 | 親環(huán)素A(CYPA)單克隆抗體 | WB |
| MAA979Mu27 | 親環(huán)素A(CYPA)單克隆抗體 | WB |
| MAA979Mu25 | 親環(huán)素A(CYPA)單克隆抗體 | WB |
| MAA979Mu26 | 親環(huán)素A(CYPA)單克隆抗體 | WB |
| MAA979Mu21 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC |
| MAA979Mu22 | 親環(huán)素A(CYPA)單克隆抗體 | WB; IHC |
| HEA979Mu | 親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA979Mu | 親環(huán)素A(CYPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA979Mu | 親環(huán)素A(CYPA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA979Mu01 | 親環(huán)素A(CYPA)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA979Mu01 | 親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Atherosclerosis | EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation[PubMed: 19758589] |
| Biomarkers | Cyclophilin-A: a novel biomarker for untreated male essential hypertension[Pubmed: 24164594] |
| Cardiovascular Diabetology | Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease[Pubmed:24502618] |
| International Journal of Biological Sciences | Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction[Pubmed:25552928] |
| Medicine | Cyclophilin A in Ruptured Intracranial Aneurysm[PubMed: 26426668] |
| Mol Cell Biochem | PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease[PubMed: 26702934] |
| Medicine (Baltimore) | Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus[PubMed: 26496315] |
| International Journal of Cardiology | Cyclophilin?A?modulates?bone?marrow-derived?CD117(+)?cells?and?enhances?ischemia-induced?angiogenesisvia?the?SDF-1/CXCR4?axis.[Pubmed:27057951] |
| Clinica?Chimica?Acta | Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor[pubmed:27823952] |
| Cardiovascular?Diabetology | Cyclophilin?A?enhances?macrophage?differentiation?and?lipid?uptake?in?high?glucose?conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.[pubmed:27809851] |
| Clinical?Science | Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity[Pubmed:29382697] |
| Journal of Molecular Histology | Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis[Pubmed:29318411] |
| Free?Radical?Research | Cyclophilin A and CD147 associate with progression of diabetic nephropathy[Pubmed: 30572748] |
| The Kaohsiung Journal of Medical Sciences | Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction[Pubmed: 31670477] |
| Cells. | Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients[Pubmed: 31936351] |
| JAMA Neurology | APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers?…[Pubmed: 24030206] |
| Menoufia Medical Journal | Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy[] |
| Toxins | Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy[34437430] |
| Cell Death & Disease | Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death[Pubmed:35732625] |