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層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Laminin (LN)

LAM

    • 層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
    • 層粘連蛋白(LN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
    • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

    特異性

    試劑盒中的抗體對層粘連蛋白(LN)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

    用法

    1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
    2.洗板1次;
    3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
    4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

    儲存

    抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

    配套試劑

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    參考文獻(xiàn)

    雜志參考文獻(xiàn)
    Stem Cell Research TherapyProtein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly[Pubmed: 24739658]
    PLoS One.Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592]
    Clin Exp Metastasis.Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression[Pubmed:24807734]
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    Archives of Plastic SurgeryComparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas[PubMed: 26430624]
    Canadian Journal of Physiology and PharmacologyModified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells[Doi: Abs]
    MOLECULAR AND CLINICAL ONCOLOGYClinical significance of serum laminin and typea??IV collagen levels in cutaneous melanoma patients[Pubmed:27330797]
    Am J Transplant.Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.[pubmed:27456745]
    Asian Pacific Journal of Cancer PreventionModulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response[pubmed:29172262]
    DiabetesCoordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes[Pubmed:29986925]
    European Food Research and TechnologyEvaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis[10.1007:s00217-018-3119-z]
    Experimental Lung ResearchHigh amplitude stretching of ATII cells and fibroblasts results in profibrotic effects[Pubmed: 31290711]
    NeurotherapeuticsFabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair[Pubmed: 31758411]
    Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellul?rmatrix von Kalbs-Stimmlippen[]
    ARCHIV?EUROMEDICACLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE[]