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E選擇素檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for E-selectin

CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E

  • E選擇素檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • E選擇素檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對E選擇素(SELE)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號適用物種：Sus scrofa; Porcine (Pig，豬)應用(僅供研究使用，不用于臨床診斷！)
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PAA029Po03E選擇素多克隆抗體WB; IHC; ICC; IP.
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KSA029Po01E選擇素檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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Journal of NeuroimmunologyEvaluation of the effects of Eserine and JWH-133 on brain dysfunction associated with experimental endotoxemia[PubMed: 25867462]
International Journal of Food Sciences and NutritionSerum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products[PubMed: 26401576]
Experimental ParasitologyAdhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis[pubmed:28821422]
journal of thoracic and cardiovascular surgeryEvaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers[Pubmed: 32812629]
BMC Endocrine DisordersThe role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children[Pubmed: 31771561]
Scientific ReportsAssociation between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea[Pubmed: 31882827]
Frontiers in Pharmacology Hyperoside Protects Human Umbilical Vein Endothelial Cells Against Anticardiolipin Antibody-Induced Injury by Activating Autophagy[Pubmed: 32508661]
Microvasc ResEffect of liraglutide on microcirculation in rat model with absolute insulin deficiency[34119534]
Anim Sci JEffect of dipeptide on intestinal peptide transporter 1 gene expression: An evaluation using primary cultured chicken intestinal epithelial cells[34309968]
Experimental and Therapeutic MedicineConstruction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis[Pubmed:35126715]