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細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白

Active Cluster Of Differentiation 147 (CD147)

EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor

  • 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白產(chǎn)品包裝(模擬)
  • 細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白產(chǎn)品包裝(模擬)
  • APB540Hu61.jpgSDS-PAGE圖
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44 66 kDa, variably N and O glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) segment and a 41 aa cytoplasmic tail. The ECD contains one C2 type and one V type Ig like domain. EMMPRIN is expressed in areas of tissue remodeling, including endometrium, placenta, skin, and regions undergoing angiogenesis. It is also expressed on cells with high metabolic activity, such as lymphoblasts, macrophages and particularly tumor cells. A functional ELISA assay was conducted to detect the interaction of recombinant Human EMMPRIN/CD147 and recombinant rat Cyclophilin B (CYPB) . Briefly, biotin-linked CD147 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CYPB-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of CD147 and CYPB was shown in Figure 1, the EC50 for this effect is 53.34 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào)適用物種:Homo sapiens (Human,人)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APB540Hu61細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白Cell?culture;?Activity?Assays.
RPB540Hu01細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB540Hu02細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPB540Hu61細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APB540Hu01細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白Cell?culture;?Activity?Assays.
APB540Hu02細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)活性蛋白Cell?culture;?Activity?Assays.
PAB540Hu01細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)多克隆抗體WB; IHC; FCM
FAB540Hu01抗細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)多克隆抗體Flow cytometry.
MAB540Hu22細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體WB; IHC; ICC/IF
MAB540Hu21細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體WB; IHC
MAB540Hu27細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體IHC
MAB540Hu24細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體IHC
MAB540Hu25細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體IHC
MAB540Hu23細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體WB
MAB540Hu28細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體WB; IHC; ICC; IP.
MAB540Hu29細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體WB; IHC; ICC; IP.
FAB540Hu02抗細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)單克隆抗體FCM
SEB540Hu細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB540Hu細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(CD147)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志參考文獻(xiàn)
DISEASE MARKERSThe Combination of CD147 and MMP-9 Serum Levels Is Identified as Novel Chemotherapy Response Markers of Advanced Non-Small-Cell Lung Cancer[Pubmed: 32377273]