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單核細胞趨化蛋白1(MCP1)活性蛋白

Active Monocyte Chemotactic Protein 1 (MCP1)

CCL2; GDCF2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; Chemokine C-C-Motif Ligand 2; Monocyte Chemotactic And Activating Factor; Monocyte Secretory Protein JE

  • 單核細胞趨化蛋白1(MCP1)活性蛋白產(chǎn)品包裝(模擬)
  • 單核細胞趨化蛋白1(MCP1)活性蛋白產(chǎn)品包裝(模擬)
  • 單核細胞趨化蛋白1(MCP1)活性蛋白Figure. Gene Sequencing (extract)
  • APA087Hu01.pngFigure. SDS-PAGE
  • 單核細胞趨化蛋白1(MCP1)活性蛋白Sample: Recombinant MCP1, Human;
    Antibody: Rabbit Anti-Human MCP1 Ab (PAA087Hu01)
    Figure. Western Blot
  • Certificate通過ISO 9001、ISO 13485質量體系認證

活性實驗

MCP-1 (monocyte chemoattractant protein 1), also known as CCL2 (C-C motif chemokine 2), is a small?cytokine?that belongs to the CC?chemokine?family. MCP-1 has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis?assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of MCP-1 on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and MCP-1 (25ng/mL and 50ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8um pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).<br/>Result: MCP-1 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (×400). Statistical result?were shown in Figure 2. The optimum chemotactic concentration of MCP-1 is about 25ng/mL, which is in accordance with references.<br/>(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 50ng/mL MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h; <br/>(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 25ng/mL MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h;<br/>(C) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without MCP-1 was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h.<br/>Figure . The chemotactic effect of MCP-1 on THP1 cells.

Figure. The chemotactic effect of MCP-1 on THP-1 cells

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。

相關產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
APA087Hu01單核細胞趨化蛋白1(MCP1)活性蛋白Cell?culture;?Activity?Assays.
RPA087Hu01單核細胞趨化蛋白1(MCP1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA087Hu01單核細胞趨化蛋白1(MCP1)多克隆抗體WB; IHC; ICC; IP.
LAA087Hu71單核細胞趨化蛋白1(MCP1)多克隆抗體(生物素標記)WB; IHC; ICC.
LAA087Hu81單核細胞趨化蛋白1(MCP1)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
MAA087Hu22單核細胞趨化蛋白1(MCP1)單克隆抗體WB; IHC; ICC; IP.
MAA087Hu24單核細胞趨化蛋白1(MCP1)單克隆抗體IHC
MAA087Hu21單核細胞趨化蛋白1(MCP1)單克隆抗體WB; IHC; ICC; IP.
LAA087Hu74單核細胞趨化蛋白1(MCP1)單克隆抗體(生物素標記)WB; IHC; ICC.
MEA087Hu單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)Enzyme-linked immunosorbent assay for Antigen Detection.
SEA087Hu單核細胞趨化蛋白1(MCP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA087Hu單核細胞趨化蛋白1(MCP1)檢測試劑盒(化學發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA087Hu單核細胞趨化蛋白1(MCP1)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
PSA087Hu01單核細胞趨化蛋白1(MCP1)抗體對ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA087Hu01單核細胞趨化蛋白1(MCP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

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