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脂質(zhì)運載蛋白2(NGAL)活性蛋白

Active Neutrophil gelatinase-associated lipocalin (NGAL)

Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

  • 脂質(zhì)運載蛋白2(NGAL)活性蛋白產(chǎn)品包裝(模擬)
  • 脂質(zhì)運載蛋白2(NGAL)活性蛋白產(chǎn)品包裝(模擬)
  • APB388Mu01.jpgFigure. SDS-PAGE
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

活性實驗

Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules. It is a 25?kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation. Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis.?Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd?= 0.41 nM). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd?values (7.9 nM). The activity assay of recombinant mouse NGAL was measured by its ability to bind Iron(III) dihydroxybenzoic acid Fe(DHBA)3. The binding of Fe(DHBA)3?results in the quenching of Trp fluorescence in Lipocalin-2. The recombinant mouse NGAL was diluted to 100 ug/ml in assay buffer (50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5). 50 μL of the different concentrations of Fe(DHBA)3 and 50 μL of 100 ug/ml rmNGAL was loaded into the plate. Incubated at room temperature for 30 minutes, then read at excitation and emission wavelengths of 280 nM and 340 nM in endpoint mode. The result was shown in Figure 1, the recombinant mouse Lipocalin-2 can bind >1 μM of Fe(DHBA)3.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號適用物種:Mus musculus (Mouse,小鼠)應(yīng)用(僅供研究使用,不用于臨床診斷!)
APB388Mu01脂質(zhì)運載蛋白2(NGAL)活性蛋白Cell?culture;?Activity?Assays.
APB388Mu61脂質(zhì)運載蛋白2(NGAL)活性蛋白Cell?culture;?Activity?Assays.
RPB388Mu02脂質(zhì)運載蛋白2(NGAL)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPB388Mu61脂質(zhì)運載蛋白2(NGAL)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB388Mu01脂質(zhì)運載蛋白2(NGAL)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB388Mu01脂質(zhì)運載蛋白2(NGAL)多克隆抗體WB; IHC
PAB388Mu02脂質(zhì)運載蛋白2(NGAL)多克隆抗體WB; IHC; ICC; IP.
LAB388Mu81脂質(zhì)運載蛋白2(NGAL)多克隆抗體(異硫氰酸熒光素標記)WB; IHC; ICC; IF.
LAB388Mu71脂質(zhì)運載蛋白2(NGAL)多克隆抗體(生物素標記)WB; IHC; ICC.
SEB388Mu脂質(zhì)運載蛋白2(NGAL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCB388Mu脂質(zhì)運載蛋白2(NGAL)檢測試劑盒(化學發(fā)光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMB388Mu脂質(zhì)運載蛋白2(NGAL)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSB388Mu01脂質(zhì)運載蛋白2(NGAL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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