血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)
ADMP-2; Aggrecanase-2
- 編號KSK205Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.312-20ng/mL
- 可適靈敏度0.112ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產品包裝(模擬)
產品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)具有高靈敏度和高特異性，經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPK205Hu01 | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAK205Hu01 | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)多克隆抗體 | WB; IHC; ICC; IP. |
| MAK205Hu21 | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)單克隆抗體 | WB; IHC; ICC; IP. |
| SEK205Hu | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMK205Hu | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSK205Hu01 | 血小板反應蛋白解整合素金屬肽酶5(ADAMTS5)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Osteoarthritis and Cartilage | Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium[PubMed: 26521751] |
| Journal of Cellular and Molecular Medicine | VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts[Pubmed:26818776] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| The American journal of pathology | Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage.[pubmed:27449198] |
| PLoS One. | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.[pubmed:28207788] |
| PLoS One | Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders[10.1371:journal.pone.0172181] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| BMC musculoskeletal disorders | Hemoglobin stimulates the expression of ADAMTS-5 and ADAMTS-9 by synovial cells: a possible cause of articular cartilage damage after intra-articular hemorrhage.[pubmed:29137610] |
| Journal of Clinical Medicine | The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma?…[Pubmed:35407621] |