α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Alpha-Tocopherol (TCPa)
VE; Vitamin E; α-TCP; α-Tocopherol
- 編號(hào)KSA922Ge11
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.23-100ug/mL
- 可適靈敏度0.46ug/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)α-生育酚(TCPa)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA922Ge11 | α-生育酚(TCPa)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA922Ge21 | α-生育酚(TCPa)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA922Ge01 | α-生育酚(TCPa)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA922Ge71 | α-生育酚(TCPa)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| CEA922Ge | α-生育酚(TCPa)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA922Ge | α-生育酚(TCPa)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA922Ge | α-生育酚(TCPa)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA922Ge11 | α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Advanced Research | Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile Men[Com:Source] |
| Journal of Physiology and Pharmacology | Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats.[PubMed: 25903950] |
| Mycotoxin Res? | The?potential?effects?of?antioxidant?feed?additives?in?mitigating?the?adverse?effects?of?cornnaturally?contaminated?with?Fusarium?mycotoxins?on?antioxidant?systems?in the intestinal mucosa, plasma, and liver in weaned pigs.[Pubmed:27021614] |
| The Journal of Nutritional Biochemistry | Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes[pubmed:27816814] |
| Plant Physiol Biochem | Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses[pubmed:29102903] |
| PLoS One | Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism[Pubmed: 30973911] |
| Prooxidant effect of α-tocopherol in pancreatic tumors.[] | |
| Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study[] |