骨成型蛋白2(BMP2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Bone Morphogenetic Protein 2 (BMP2)
BMP2A; BMP-2A; Hemochromatosis Modifier
- 編號KSA013Mu01
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍7.81-500pg/mL
- 可適靈敏度3.3pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對骨成型蛋白2(BMP2)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA013Mu02 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Mu01 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA013Mu02 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA013Mu01 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA013Mu71 | 骨成型蛋白2(BMP2)多克隆抗體(生物素標記) | WB |
| LAA013Mu81 | 骨成型蛋白2(BMP2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SEA013Mu | 骨成型蛋白2(BMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA013Mu | 骨成型蛋白2(BMP2)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA013Mu | 骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA013Mu01 | 骨成型蛋白2(BMP2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice[Wiley: Source] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Medical Journal of Chinese People's Liberation Army | Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion[Source] |
| PLOS ONE | PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling[Pubmed:25470749] |
| Eur J Pharmacol | Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy[PubMed: 25934570] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy[PubMed: 25871861] |
| Journal of Cellular Biochemistry | Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway[PubMed: 26681207] |
| International Journal of Nanomedicine | Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis[Pubmed:27042064] |
| Research report | EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO[files:p013-022] |
| Gene? | Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE[Pubmed:27196063] |
| Journal of Materials Chemistry B | Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair[DOI:10.1039/C7TB00640C] |
| Scientific Reports | 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering.[pubmed:29042614] |
| Journal of Applied Biomaterials & Functional Materials | Demineralized dentin and enamel matrices as suitable substrates for bone regeneration[pubmed:28731486] |
| Journal of?Clinical?Periodontology? | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats[Pubmed:29574908] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats[Pubmed:29898457] |
| Journal of?Applied?Biomaterials?&?Functional?Materials | BMP-2 and type I collagen preservation in human deciduous teeth after demineralization[Pubmed:30045659] |
| Applied Surface Science | Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction[] |
| Materials Science and Engineering: C | Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration[] |
| Biomaterials Science | Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration[] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats[Pubmed: 32004919] |
| Journal of Industrial and Engineering Chemistry | Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration[] |
| BMC Musculoskeletal Disorders | Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse[] |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density[33842739] |
| Tissue Eng Regen Med | Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro[34302695] |
| Toxicon | The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats[34742779] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum[34472855] |
| Phytomedicine | Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane[Pubmed:35278899] |