促腎上皮質激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Corticotropin Releasing Factor (CRF)
CRH; Corticotropin Releasing Hormone; Corticoliberin
- 編號KSA835Hu11
- 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
- 試劑內容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.91pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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特異性
試劑盒中的抗體對促腎上皮質激素釋放激素(CRH)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA835Hu04 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA835Hu03 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA835Hu01 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA835Hu02 | 促腎上皮質激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA835Hu24 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA835Hu25 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA835Hu21 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA835Hu22 | 促腎上皮質激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA835Hu33 | 促腎上皮質激素釋放激素(CRF)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA835Hu01 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA835Hu03 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA835Hu02 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA835Hu04 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB |
PAA835Hu07 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA835Hu08 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
PAA835Hu09 | 促腎上皮質激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
LAA835Hu81 | 促腎上皮質激素釋放激素(CRF)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAA835Hu71 | 促腎上皮質激素釋放激素(CRF)多克隆抗體(生物素標記) | WB |
MAA835Hu22 | 促腎上皮質激素釋放激素(CRF)單克隆抗體 | WB; IHC; ICC; IP. |
MAA835Hu21 | 促腎上皮質激素釋放激素(CRF)單克隆抗體 | WB |
CEA835Hu | 促腎上皮質激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA835Hu | 促腎上皮質激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA835Hu11 | 促腎上皮質激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats[Pubmed:24440345] |
Bipolar?Disord. | Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?[Pubmed:24862833] |
Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level[Pubmed:25236411] |
Ann Dermatol | Acute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth.[pubmed:27746640] |
Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation[pubmed:28663093] |
Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation[pubmed:29082242] |
Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats[Pubmed:30028991] |
Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?…[Pubmed:30038568] |
Toxicology Letters | Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors[Pubmed:29807116] |
Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?…[Pubmed:29379286] |
BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?…[Pubmed:29540178] |
Bioscience Research | Bioscience Research[] |
FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice[Pubmed: 31431066] |
Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging[Pubmed: 32463582] |
Endocr J | Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress[33408312] |
CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress[33651993] |