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27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Heat Shock Protein 27 (Hsp27)

HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

  • 27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 27kDa熱休克蛋白(Hsp27)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)27kDa熱休克蛋白(HSPB1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào)適用物種：Homo sapiens (Human，人)應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

雜志參考文獻(xiàn)
Animal Science JournalThe effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells[PubMed: 26467738]
Nature?CommunicationsHSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis[Pubmed:29650953]
Advances in Experimental?Medicine?and?BiologyMolecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease[Pubmed:29429028]
Diabetes Research and Clinical PracticeThe beneficial effects of 15 units of high-intensity circuit training in women is modified by age, baseline insulin resistance and physical capacity[Pubmed: 31102684]
Journal of Immunology ResearchDamage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[]
Journal of Clinical MedicineDamage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort[Pubmed: 32210109]
MedicineRegulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771]
Beilstein ArchivesImmunization of mice with gold nanoparticles conjugated to thermostable tumor antigens prevents tumor development during transplantation[]