骨橋素(OPN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- 編號(hào)KSA899Ca01
- 物種Canis familiaris; Canine (Dog，犬)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.312-20ng/mL
- 可適靈敏度0.117ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價(jià)格¥ 7776¥ 12960¥ 23328¥ 45360¥ 77760
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)骨橋素(OPN)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA899Ca01 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA899Ca02 | 骨橋素(OPN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA899Ca01 | 骨橋素(OPN)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA899Ca71 | 骨橋素(OPN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| HEA899Ca | 骨橋素(OPN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA899Ca | 骨橋素(OPN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA899Ca | 骨橋素(OPN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA899Ca01 | 骨橋素(OPN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Biomedical Materials Research Part A | Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture[PubMed: 19280635] |
| Osteoarthritis and Cartilage | Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients[PubMed: 19747583] |
| The Journal of Rheumatology | Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis.[Jrheum: source] |
| International Journal of Pharmaceutics | Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)[ScienceDirect: S0378517312003742] |
| European Cells and matreials | The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering[Ecmjournal: Source] |
| Journal of Equine Veterinary Science | Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse[ScienceDirect: Source] |
| Basic & Clinical Pharmacology & Toxicology | Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions[Wiley:Source] |
| Journal of Cellular Biochemistry | Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts[Pubmed:24905552] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats[Pubmed:25038619] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders[Pubmed:25245337] |
| FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells[Pubmed:25542352] |
| Gene | Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis[PubMed: 26283150] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer[PubMed: 26266370] |
| Journal of Biomaterials and Tissue Engineering | Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel[Content: Asp] |
| Primasari | Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer[Index.Php: Amor] |
| Int J Biol Sci | Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway[PubMed: 26327817] |
| Journal of Kunming Medical University / Kunming Yike Daxue Xuebao | Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma.[Ebscohost] |
| Am J Pathol.? | Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.[Pubmed:26963343] |
| circulation journal | Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification.[pubmed:28344201] |
| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.[pubmed:28127817] |
| American Journal of Translational Research | Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes.[pubmed:28337262] |
| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans[pubmed:29212824] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.[pubmed:28742995] |
| Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720] |
| Indian?Journal?of?Nephrology | Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease[id:233806] |
| Carbon | In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells[Doi: 10.1016/j.carbon.2018.10.081] |
| current?eye?research | Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma.[Pubmed: 30994369] |
| Journal of Proteomics | Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma[Pubmed: 31629959] |
| Acta Biomaterialia | Inflammatory response to magnesium-based biodegradable implant materials[Pubmed: 31610341] |
| Journal of Clinical Medicine | Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …[Pubmed: 32182704] |
| EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats[Pubmed: 33010420] |
| [ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials[] |
| Biomolecules | Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44[34070790] |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells[] | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?…[34857049] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study[Pubmed:35743980] |