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血小板衍生生長(zhǎng)因子A(PDGFA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Platelet Derived Growth Factor Subunit A (PDGFA)

PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide

  • 血小板衍生生長(zhǎng)因子A(PDGFA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 血小板衍生生長(zhǎng)因子A(PDGFA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)血小板衍生生長(zhǎng)因子A(PDGFA)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

參考文獻(xiàn)

雜志參考文獻(xiàn)
Canadian Journal of Physiology and PharmacologyThe effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats[pubmed:28177680]
International ImmunopharmacologyShikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway[pubmed:28521243]
Open Access Macedonian Journal of Medical SciencesLow Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells[]
Prostaglandins & other Lipid MediatorsLipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway[Pubmed: 31698141]
Ann Agric Environ MedPDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis[Pubmed: 32208584]
Tissue Eng Regen MedEffect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method[34339025]
Biomed Research InternationalThermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form[Pubmed:35586817]