抗增殖細胞核抗原(PCNA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 編號KSA591Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.059ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對抗增殖細胞核抗原(PCNA)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA591Hu01 | 抗增殖細胞核抗原(PCNA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAA591Hu01 | 抗抗增殖細胞核抗原(PCNA)多克隆抗體 | WB; IHC |
| PAA591Hu01 | 抗增殖細胞核抗原(PCNA)多克隆抗體 | WB; IHC |
| MAA591Hu21 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC/IF |
| MAA591Hu22 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB |
| MAA591Hu23 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB |
| MAA591Hu25 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB |
| MAA591Hu24 | 抗增殖細胞核抗原(PCNA)單克隆抗體 | WB |
| CAA591Hu22 | 抗抗增殖細胞核抗原(PCNA)單克隆抗體 | WB; IHC; ICC/IF |
| FAA591Hu02 | 抗抗增殖細胞核抗原(PCNA)單克隆抗體 | FCM |
| FAA591Hu04 | 抗抗增殖細胞核抗原(PCNA)單克隆抗體 | FCM |
| KSA591Hu01 | 抗增殖細胞核抗原(PCNA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation[34415018] |
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| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[] | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?…[] |