凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Prothrombin Fragment 1+2 (F1+2)
- 編號KSA710Hu01
- 物種Homo sapiens (Human,人)相同的名稱,不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.78 -50ng/mL
- 可適靈敏度0.36ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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特異性
試劑盒中的抗體對凝血酶原片段F1+2(F1+2)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA710Hu01 | 凝血酶原片段F1+2(F1+2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA710Hu01 | 凝血酶原片段F1+2(F1+2)多克隆抗體 | WB; IHC; ICC; IP. |
LAA710Hu71 | 凝血酶原片段F1+2(F1+2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA710Hu22 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC |
MAA710Hu23 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC; ICC/IF |
MAA710Hu24 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC |
MAA710Hu25 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC; ICC; IP. |
MAA710Hu21 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC; ICC; IP. |
MAA710Hu26 | 凝血酶原片段F1+2(F1+2)單克隆抗體 | WB; IHC; ICC; IP. |
FAA710Hu02 | 抗凝血酶原片段F1+2(F1+2)單克隆抗體 | Flow cytometry. |
LAA710Hu72 | 凝血酶原片段F1+2(F1+2)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA710Hu | 凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA710Hu | 凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA710Hu | 凝血酶原片段F1+2(F1+2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA710Hu | 凝血酶原片段F1+2(F1+2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA710Hu01 | 凝血酶原片段F1+2(F1+2)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA710Hu01 | 凝血酶原片段F1+2(F1+2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
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Journal of Vascular Surgery | Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair[ScienceDirect: S0741521411018568] |
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine | High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study[Sjtrem:1757-7241] |
Journal of Thrombosis and Haemostasis | High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality[Wiley: source] |
British Journal of Dermatology | Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria[PubMed: 21910697] |
Arteriosclerosis, Thrombosis, and Vascular Biology. | Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status[PubMed: 22859493] |
Journal of Vascular Surgery | Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis[PubMed: 23140799] |
PLoS ONE | Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension[Plosone: Source] |
Journal of Vascular Surgery | Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis[Pubmed: 23140799 ] |
Haemetology | Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India[Pubmed: 23992124] |
Cogent Medicine | Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction[Tandfonline:Source] |
Interact Cardiovasc Thorac Surg. | An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose[Pubmed:24632424] |
Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing[PubMed: 26243277] |
J Thromb Haemost | Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism[PubMed: 26514101] |
Acta Oncologica Turcica | Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer [aot:AOT_49_1_6_12.pdf] |
Advances in Medical Sciences | Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation[pubmed:29145170] |
Thrombosis Research | The causes of thrombocytopenia after transcatheter aortic valve implantation[pubmed:28582640] |
Acta?Haematologica | Elevations of Thrombotic Biomarkers in Hemoglobin H Disease[Pubmed:29402840] |
Journal of Neurotrauma | The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice[Pubmed: 31830857] |
Indian Journal of Public Health Research & Development | Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever[] |
Rheumatol Int | The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary?…[Pubmed:35284968] |