Salusin肽β(SALb)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Salusin Beta (SALb)
- 編號KSC026Mu11
- 物種Mus musculus (Mouse,小鼠)相同的名稱,不同的物種。
- 試劑內容包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍4.94-400pg/mL
- 可適靈敏度1.92pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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特異性
試劑盒中的抗體對Salusin肽β(SALb)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
CPC026Mu21 | Salusin肽β(SALb)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
CEC026Mu | Salusin肽β(SALb)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC026Mu | Salusin肽β(SALb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSC026Mu11 | Salusin肽β(SALb)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of Maternal-Fetal and Neonatal Medicine | Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia[Informa: 14767058.2012.660221] |
Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass[PubMed: 22884920] |
Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows[ScienceDirect: S0196978113000533] |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses.[PubMed: 23178004] |
Biotech Histochem. | Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose.[PubMed: 23244237] |
Gynecological Endocrinology, | Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome.[PubMed: 22827297] |
Peptides | Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus[ScienceDirect: S0196978113003100] |
Biotechnic & Histochemistry | Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome [Com:Source] |
Med Sci Monit. | Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome[Pubmed:Pmc4136931] |
Acta Physiologica | Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats[Pubmed:24304512] |
Pediatr Nephrol. | Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population?[Pubmed:25245503] |
European Journal of Dermatology | Serum salusin-α and salusin-β levels in patients with psoriasis[PubMed: 26055619] |
J Investig Med | Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease[PubMed: 25730454] |
Genet Test Mol Biomarkers.? | Serum Salusin-β Levels Are Correlated with Slow Coronary Flow.[Pubmed:27227272] |
Sci Rep.? | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats.[pubmed:28230187] |
Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy[pubmed:28333148] |
Oxidative Medicine and Cellular Longevity | Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma[10.1155:2017] |
International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus[Pubmed: 30581101] |
Multiple Sclerosis and?Related?Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis[Pubmed: 30738876] |
cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity[Pubmed: 31486354] |
Redox Biology | Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway[Pubmed: 31884071] |
Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis[Pubmed: 32054363] |
cardiovascular drugs and therapy | A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats.[Pubmed: 32458319] |
Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors[] | |
Mol Med Rep | Salusin?β participates in high glucose?induced HK?2 cell ferroptosis in a Nrf?2?dependent manner[34296310] |