腫瘤壞死因子β(TNFb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Tumor Necrosis Factor Beta (TNFb)
LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
- 編號KSA134Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍7.8-500pg/mL
- 可適靈敏度3.3pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
- 價格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對腫瘤壞死因子β(TNFb)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA134Ra01 | 腫瘤壞死因子β(TNFb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA134Ra02 | 腫瘤壞死因子β(TNFb)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA134Ra02 | 腫瘤壞死因子β(TNFb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA134Ra01 | 腫瘤壞死因子β(TNFb)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA134Ra02 | 腫瘤壞死因子β(TNFb)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA134Ra21 | 腫瘤壞死因子β(TNFb)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA134Ra | 腫瘤壞死因子β(TNFb)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA134Ra | 腫瘤壞死因子β(TNFb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA134Ra01 | 腫瘤壞死因子β(TNFb)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| CGA134Ra02 | 腫瘤壞死因子β(TNFb)cDNA | PCR、蛋白表達等 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Applied Physiology | Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process[PubMed: 22984252] |
| Journal of Translational Medicine | Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation[Pubmed:26847569] |
| International Journal of Stem Cell Research and Transplantation | Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines[IJST-2328-3548-04-803] |
| Probiotics Antimicrob Proteins | Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a?…[Pubmed:29305686] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?…[Pubmed: 32680553] |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort[] | |
| BMC MUSCULOSKELETAL DISORDERS | Elevated lymphotoxin-|á (TNF|?) is associated with intervertebral disc degeneration[33441130] |