8-羥基脫氧鳥苷(8-OHdG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)
8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG
- 編號CEA660Ge
- 物種Pan-species (General，通用)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍74.07-6,000pg/mL
- 靈敏度最小可檢測劑量小于等于26.81pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3342¥ 4774¥ 21481¥ 40576¥ 334152
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測8-羥基脫氧鳥苷(8-OHdG)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的8-羥基脫氧鳥苷(8-OHdG)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-90 | 87 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 89-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的8-羥基脫氧鳥苷(8-OHdG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中8-羥基脫氧鳥苷(8-OHdG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-88% | 98-105% | 78-98% | 84-91% |
| EDTA plasma(n=5) | 95-103% | 96-103% | 87-94% | 89-97% |
| heparin plasma(n=5) | 80-92% | 79-101% | 83-92% | 80-91% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將8-羥基脫氧鳥苷(8-OHdG)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CEA660Ge | 8-羥基脫氧鳥苷(8-OHdG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA660Ge | 8-羥基脫氧鳥苷(8-OHdG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA660Ge | 8-羥基脫氧鳥苷(8-OHdG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Yonsei Med J. | Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients.[Pubmed: 24142649] |
| J Histochem Cytochem | Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats[PubMed: 26385569] |
| Diabetes/Metabolism Research and Reviews | Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of …[doi:10.1002] |
| Nutrition Journal | Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study[Pubmed:27071648] |
| Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection[pmc:PMC4855822] |
| UVaDOC | Dieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sue?o. Significado de la leptina en el control respiratorio[Tesis1149-161108.pdf] |
| Journal of Diabetes Mellitus | The Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia[profile:Wesam_Nasef3] |
| Indian Journal of Hematology and Blood Transfusion | Oleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury[article:10.1007] |
| Journal?of?Diabetes?Research | Oxidative?Damage?to the?Salivary?Glands?of?Rats?with?Streptozotocin-Induced?Diabetes-TemporalStudy:?Oxidative?Stress?and?Diabetic?Salivary?Glands.[pubmed:27478848] |
| Front Physiol. | Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats.[pubmed:28184199] |
| Archives of Oral Biology | Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats[pubmed:28926744] |
| BioMed Research International | Oxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery[10.1155:2017] |
| Oncotarget | Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway[pubmed:29100404] |
| International Journal of Molecular Sciences | Antioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia?[pubmed:29053628] |
| Antioxidants and Redox Signaling | Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury[10.1089:ars.2016.6758] |
| Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats[ISSN:1940-5901/IJCEM0048822] | |
| Biological Trace Element Research | Protective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats[pubmed:28616770] |
| HAYATI Journal of Biosciences | Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult SpragueeDawley Rats[10.1016/j.hjb.2017.08.007] |
| Scientific Reports | Risk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoea[pubmed:29234143] |
| Journal of Pain Research | Sex differences in complex regional pain syndrome type I (CRPS-I) in mice[pubmed:28831269] |
| HAYATI Journal of Biosciences | Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley Rats[10.1016/j.hjb.2017.08.007] |
| Oxidative Medicine and Cellular Longevity | Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance[Pubmed:29636851] |
| Oxidative medicine and cellular longevity | Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures[Pubmed:30013720] |
| Journal of Cardiovascular Development and Disease | Oxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic Subjects[Pubmed:29518984] |
| Reproductive?Biology | Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring[Pubmed:30005909] |
| Oxidative medicine and cellular longevity | Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance[] |
| Journal of Pharmacological Reports | Journal of Pharmacological Reports[Pubmed: 27489887] |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Cross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetes[] |
| BioMed Research International | Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet?…[] |
| Oxidative Medicine and Cellular Longevity | Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function[] |
| Oxidative Medicine and Cellular Longevity | Dendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1[] |
| International Journal of Pharmaceutical Research & Allied Sciences | Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats.[] |
| Journal of Diabetes and Metabolism | Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients[] |
| Journal of Food Biochemistry | Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic?…[Pubmed: 31373029] |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains[Pubmed: 31468353] |
| Oxidative Medicine and Cellular Longevity | Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population[Pubmed: 32215181] |
| Journal of Clinical Medicine | Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents[Pubmed: 32079369] |
| Frontiers in Pharmacology | Hydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent Manner[Pubmed: 32116706] |
| Odontology | Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis[Pubmed: 32065311] |
| FREE RADICAL RESEARCH | Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide?…[Pubmed: 32985285] |
| JOURNAL OF FOOD SCIENCE | Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress[Pubmed: 32885410] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
| Lipids Health Dis | Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism …[Pubmed: 32487177] |
| Theranostics | Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis[Pubmed: 32483416] |
| Journal of Clinical Medicine | Systemic Redox Imbalance in Patients with Chronic Granulomatous Disease[Pubmed: 32397350] |
| Oxidative Medicine and Cellular Longevity | NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General?…[Pubmed: 33274008] |
| OXIDATIVE MEDICINE AND CELLULAR LONGEVITY | Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross …[Pubmed: 32509152] |
| oxidative medine cellular longevity | Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β …[Pubmed: 32617139] |
| MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workers[Pubmed: 32928371] |
| Journal of Functional Foods | Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets[] |
| Journal of Diabetes Research | Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China[] |
| The potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosis[] | |
| Mutat Res Gen Tox En[] | |
| Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord …[] | |
| Journal of Skin and Stem Cell | 8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment[] |
| Redox Biol | Biomarkers of nucleic acid oxidation¨Ca summary state-of-the-art[33579665] |
| Theranostics | Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage[33897894] |
| Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats[34093766] |
| chemosphere | Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective[33662726] |
| Food Funct. | Sestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injury[33900303] |
| Aquaculture Reports | Growth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to??-[] |
| Ann Behav Med | Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial[34180500] |
| Cancer Manag Res | Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer[34408493] |
| The Journal of Clinical Endocrinology & Metabolism | High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα[34562085] |
| Ecotoxicol Environ Saf | Chromium induced neurotoxicity by altering metabolism in zebrafish larvae[34781135] |
| Toxins | Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy[34437430] |
| J Int Med Res | Body mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese population[Pubmed:35209724] |
| Ann Transplant | Biochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients[Pubmed:34751188] |
| Oxidative Medicine and Cellular Longevity | Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway[Pubmed:35602095] |
