抗免疫球蛋白A抗體(Anti-IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Anti-Immunoglobulin A Antibody (Anti-IgA)
- 編號AEA546Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法間接法
- 反應(yīng)時長2h, 30min
- 檢測范圍6.25-400ng/mL
- 靈敏度最小可檢測劑量小于等于2.52ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3360¥ 4800¥ 21600¥ 40800¥ 336000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標(biāo)準(zhǔn)曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測抗免疫球蛋白A抗體(Anti-IgA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗免疫球蛋白A抗體(Anti-IgA)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 84-95 | 87 |
| heparin plasma(n=5) | 85-94 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗免疫球蛋白A抗體(Anti-IgA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抗免疫球蛋白A抗體(Anti-IgA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-101% | 79-92% | 83-98% | 88-102% |
| EDTA plasma(n=5) | 83-101% | 92-101% | 92-105% | 96-104% |
| heparin plasma(n=5) | 98-105% | 95-104% | 81-95% | 78-89% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的抗免疫球蛋白A抗體(Anti-IgA)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗免疫球蛋白A抗體(Anti-IgA)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA546Hu01 | 免疫球蛋白A(IgA)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA546Hu05 | 免疫球蛋白A(IgA)多克隆抗體 | WB; IHC |
| PAA546Hu01 | 免疫球蛋白A(IgA)多克隆抗體 | IHC |
| SAA546Hu09 | 兔抗人IgA多克隆抗體(辣根過氧化物酶標(biāo)記) | ELISA; WB; IHC; ICC. |
| LAA546Hu71 | 免疫球蛋白A(IgA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA546Hu | 免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA546Hu | 免疫球蛋白A(IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA546Hu | 抗免疫球蛋白A抗體(Anti-IgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA546Hu | 免疫球蛋白A(IgA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA546Hu | 免疫球蛋白A(IgA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA546Hu01 | 免疫球蛋白A(IgA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
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