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和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Copeptin (CPP)

C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

    • 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
    • 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
    • 和肽素(CPP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)實驗結(jié)果圖
    • SEA365Hu.jpg標(biāo)準(zhǔn)曲線圖
    • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

    特異性

    本試劑盒用于檢測和肽素(CPP),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
    由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

    回收率

    分別于定值血清及血漿樣本中加入一定量的和肽素(CPP)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

    樣本回收率范圍(%)平均回收率(%)
    serum(n=5)97-105101
    EDTA plasma(n=5)89-10398
    heparin plasma(n=5)81-9787

    精密度

    精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
    批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
    批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
    批內(nèi)差: CV<10%
    批間差: CV<12%

    線性

    在定值血清及血漿樣本內(nèi)加入適量的和肽素(CPP),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中和肽素(CPP)含量的測定值與理論值的比率。

    樣本1:21:41:81:16
    serum(n=5)80-94%79-101%92-101%81-90%
    EDTA plasma(n=5)96-105%85-101%80-103%97-104%
    heparin plasma(n=5)89-96%88-103%87-101%89-99%

    穩(wěn)定性

    經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
    為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。

    實驗流程

    1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
    2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時;
    3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
    4. 洗板3次;
    5. 加檢測溶液B100µL,37°C孵育30分鐘;
    6. 洗板5次;
    7. 加TMB底物90µL,37°C孵育10-20分鐘;
    8. 加終止液50µL,立即450nm讀數(shù)。

    實驗原理

    將和肽素(CPP)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的和肽素(CPP)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的和肽素(CPP)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的和肽素(CPP)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

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    參考文獻(xiàn)

    雜志參考文獻(xiàn)
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