血紅素氧合酶1(HO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Heme Oxygenase 1 (HO1)
HMOX1; Hsp32; HMOX1D
- 編號SEA584Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.115ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 2646¥ 3780¥ 17010¥ 32130¥ 264600
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測血紅素氧合酶1(HO1)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血紅素氧合酶1(HO1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 97 |
| EDTA plasma(n=5) | 87-101 | 97 |
| heparin plasma(n=5) | 85-101 | 89 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血紅素氧合酶1(HO1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血紅素氧合酶1(HO1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-101% | 90-98% | 84-92% | 88-103% |
| EDTA plasma(n=5) | 78-98% | 89-99% | 93-101% | 98-105% |
| heparin plasma(n=5) | 81-94% | 91-103% | 94-101% | 90-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將血紅素氧合酶1(HO1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的血紅素氧合酶1(HO1)與連接于固相載體上的抗體結合，然后加入生物素化的血紅素氧合酶1(HO1)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的血紅素氧合酶1(HO1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA584Hu01 | 血紅素氧合酶1(HO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA584Hu02 | 血紅素氧合酶1(HO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA584Hu01 | 血紅素氧合酶1(HO1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA584Hu02 | 血紅素氧合酶1(HO1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA584Hu71 | 血紅素氧合酶1(HO1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA584Hu81 | 血紅素氧合酶1(HO1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA584Hu21 | 血紅素氧合酶1(HO1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA584Hu22 | 血紅素氧合酶1(HO1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA584Hu | 血紅素氧合酶1(HO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA584Hu | 血紅素氧合酶1(HO1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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| brain research | Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling.[pubmed:28238669] |
| biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats.[pubmed:28068636] |
| Inflammopharmacology. | Implication of Nrf2/HO-1 pathway in the coloprotective effect of coenzyme Q10 against experimentally induced ulcerative colitis.[pubmed:28050757] |
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| Journal of Microbiology, Immunology and Infection | Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients[pubmed:28655573] |
| Nutrition & Metabolism | Hepatic, adipocyte, enteric and pancreatic hormones: response to dietary macronutrient composition and relationship with metabolism[pubmed:28694840] |
| Biomedicine & Pharmacotherapy | Ameliorative potential of sitagliptin and/or resveratrol on experimentallyinduced clear cell renal cell carcinoma[pubmed:29101811] |
| Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children[DOI:?10.1111/apa.14106] |
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| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms[pubmed:28061403] |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?…[10.1016:j.talanta.2018.01.044] |
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| Journal of?Infection?and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity[Pubmed:29753615] |
| American Institute of Physics | Anti-hemolytic and anti-inflammatory activities of the methanolic extract of (P.Beauv.) Briq. leaves in 2-butoxyethanol-hemolytic induced?…[10.1063:1.5033415] |
| Iranian?Journal?of?Basic?Medical Sciences | Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress[Doi: 10.22038/IJBMS.2018.31716.7714] |
| Biomedicine & Pharmacotherapy | Targeting Nrf2/HO-1 signaling by crocin: Role in attenuation of AA-induced ulcerative colitis in rats[Pubmed: 30530041] |
| Experimental Parasitology | Sodium arsenite augments sensitivity of Echinococcus granulosus protoscoleces to albendazole[Pubmed: 30790573] |
| JGH OPEN | Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis[] |
| Journal of Food Biochemistry | Vanillic acid alleviates palmitic acid‐induced oxidative stress in human umbilical vein endothelial cells via Adenosine Monophosphate‐Activated Protein Kinase?…[] |
| Experimental and Therapeutic Medicine | Effect of heme oxygenase 1 and renin/prorenin receptor on oxidized low?density lipoprotein?induced human umbilical vein endothelial cells[] |
| Life Sciences | Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis[Pubmed: 31465731] |
| Respiratory physiology & neurobiology | Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosis[Pubmed: 31600583] |
| Life Sci | The protective effect of Lactobacillus versus 5-aminosalicylic acid in ulcerative colitis model by modulation of gut microbiota and Nrf2/Ho-1 pathway[Pubmed: 32526285] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment[Pubmed: 28593326] |
| JOURNAL OF PHARMACY AND PHARMACOLOGY | Isoliquiritigenin attenuates inflammation and modulates Nrf2/caspase-3 signalling in STZ-induced aortic injury[33793806] |
| Exp Parasitol | In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism[Pubmed:35594934] |
| Free Radical Biology and Medicine | Flavonoid 4, 4′-dimethoxychalcone induced ferroptosis in cancer cells by synergistically activating Keap1/Nrf2/HMOX1 pathway and inhibiting FECH[Pubmed:35697292] |
