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脂蛋白關聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Phospholipase A2 Group VII (LpPLA2)

PLA2G7; PAF-AH; PAFAH; Lp-PLA2; LDL-PLA2; Platelet Activating Factor Acetylhydrolase,Plasma; Phospholipase A2,Group VII; LDL-associated phospholipase A2; Phospholipase A2, Lipoprotein Associated

  • 脂蛋白關聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 脂蛋白關聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 脂蛋白關聯(lián)磷脂酶A2(LpPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)實驗結果圖
  • SEA867Hu.jpg標準曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測脂蛋白關聯(lián)磷脂酶A2(LpPLA2),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質(zhì)交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的脂蛋白關聯(lián)磷脂酶A2(LpPLA2)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)96-105101
EDTA plasma(n=5)78-10491
heparin plasma(n=5)85-9691

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的脂蛋白關聯(lián)磷脂酶A2(LpPLA2),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中脂蛋白關聯(lián)磷脂酶A2(LpPLA2)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)82-91%89-99%83-98%96-104%
EDTA plasma(n=5)98-105%89-97%78-101%78-94%
heparin plasma(n=5)78-95%83-97%78-102%94-101%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將脂蛋白關聯(lián)磷脂酶A2(LpPLA2)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的脂蛋白關聯(lián)磷脂酶A2(LpPLA2)與連接于固相載體上的抗體結合,然后加入生物素化的脂蛋白關聯(lián)磷脂酶A2(LpPLA2)抗體,將未結合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的脂蛋白關聯(lián)磷脂酶A2(LpPLA2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產(chǎn)品

編號適用物種:Homo sapiens (Human,人)應用(僅供研究使用,不用于臨床診斷!)
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