谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Glutathione Peroxidase 1 (GPX1)
GSHPX1; Cellular glutathione peroxidase
- 編號SEA295Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.13ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結(jié)果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測谷胱甘肽過氧化酶1(GPX1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的谷胱甘肽過氧化酶1(GPX1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 95 |
| EDTA plasma(n=5) | 89-96 | 92 |
| heparin plasma(n=5) | 91-98 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的谷胱甘肽過氧化酶1(GPX1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中谷胱甘肽過氧化酶1(GPX1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-104% | 82-93% | 81-101% | 94-103% |
| EDTA plasma(n=5) | 98-105% | 95-102% | 81-94% | 89-103% |
| heparin plasma(n=5) | 89-103% | 94-103% | 80-101% | 80-91% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將谷胱甘肽過氧化酶1(GPX1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的谷胱甘肽過氧化酶1(GPX1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的谷胱甘肽過氧化酶1(GPX1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的谷胱甘肽過氧化酶1(GPX1)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA295Mu01 | 谷胱甘肽過氧化酶1(GPX1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA295Mu02 | 谷胱甘肽過氧化酶1(GPX1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA295Mu02 | 谷胱甘肽過氧化酶1(GPX1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA295Mu01 | 谷胱甘肽過氧化酶1(GPX1)多克隆抗體 | WB; IHC |
| SEA295Mu | 谷胱甘肽過氧化酶1(GPX1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA295Mu | 谷胱甘肽過氧化酶1(GPX1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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