甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- 編號SEA819Bo
- 物種Bos taurus; Bovine (Cattle，牛)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.8pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3175¥ 4536¥ 20412¥ 38556¥ 317520
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測甲狀旁腺激素相關蛋白(PTHrP)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的甲狀旁腺激素相關蛋白(PTHrP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 83-91 | 87 |
| EDTA plasma(n=5) | 84-91 | 87 |
| heparin plasma(n=5) | 89-97 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的甲狀旁腺激素相關蛋白(PTHrP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中甲狀旁腺激素相關蛋白(PTHrP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-97% | 79-101% | 88-96% | 83-92% |
| EDTA plasma(n=5) | 96-103% | 91-103% | 94-105% | 95-105% |
| heparin plasma(n=5) | 91-98% | 98-105% | 98-105% | 81-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將甲狀旁腺激素相關蛋白(PTHrP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的甲狀旁腺激素相關蛋白(PTHrP)與連接于固相載體上的抗體結合，然后加入生物素化的甲狀旁腺激素相關蛋白(PTHrP)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的甲狀旁腺激素相關蛋白(PTHrP)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA819Bo01 | 甲狀旁腺激素相關蛋白(PTHrP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA819Bo01 | 甲狀旁腺激素相關蛋白(PTHrP)多克隆抗體 | WB |
| MAA819Bo21 | 甲狀旁腺激素相關蛋白(PTHrP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA819Bo | 甲狀旁腺激素相關蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA819Bo | 甲狀旁腺激素相關蛋白(PTHrP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Stem Cells and Development | Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated.[PubMed: 22133004] |
| International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition[PubMed: PMC3753447] |
| Investigational New Drugs | Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts[Pubmed: 23673814] |
| Toxicology and Applied Pharmacology | Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment[Pubmed:24463094] |
| Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study[Pubmed:24943060] |
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| Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation.[Pubmed:27312487] |
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| International Journal of Molecular Sciences | A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice[Pubmed: 31600911] |
| Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses[Pubmed: 32571421] |
| International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related?…[] |
| Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни[] | |
| Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ?-[33510128] |
| Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model[34732809] |
| Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals[Pubmed:35334154] |
