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抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Proliferating Cell Nuclear Antigen (PCNA)

Cyclin

  • 抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)產(chǎn)品包裝(模擬)
  • 抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)實驗結(jié)果圖
  • SEA591Mi.jpg標準曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒對檢測抗增殖細胞核抗原(PCNA)具有較高的靈敏度。
經(jīng)檢測人、小鼠、大鼠之間有100%的交叉反映。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將抗增殖細胞核抗原(PCNA)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的抗增殖細胞核抗原(PCNA)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的抗增殖細胞核抗原(PCNA)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗增殖細胞核抗原(PCNA)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關產(chǎn)品

編號適用物種:Homo sapiens (Human,人), Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠)應用(僅供研究使用,不用于臨床診斷!)
RPA591Mi01抗增殖細胞核抗原(PCNA)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA591Mi01抗增殖細胞核抗原(PCNA)多克隆抗體WB; IHC
MAA591Mi22抗增殖細胞核抗原(PCNA)單克隆抗體WB; IHC
MAA591Mi23抗增殖細胞核抗原(PCNA)單克隆抗體WB; IHC
CAA591Mi22抗抗增殖細胞核抗原(PCNA)單克隆抗體WB
MAA591Mi24抗增殖細胞核抗原(PCNA)單克隆抗體WB; IHC; ICC; IP.
MAA591Mi21抗增殖細胞核抗原(PCNA)單克隆抗體WB; IHC; ICC/IF
SEA591Mi抗增殖細胞核抗原(PCNA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA591Mi抗增殖細胞核抗原(PCNA)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
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Life?SciencesMalaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats[Pubmed: 30205096]
Archives of Physiology and BiochemistryDiabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin[Pubmed: 30513216]
European journal of medicinal chemistrySynthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity[Pubmed: 31216504]
Antioxidants (Basel).The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets[Pubmed: 31426309]
AntioxidantsOxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian?…[Pubmed: 31600920]
TOXICOLOGYTert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis[Pubmed: 32565124]
BIOMEDICINE & PHARMACOTHERAPYProtective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats[Pubmed: 33152939]
Sci RepTert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats[33750916]
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Naunyn-Schmiedeberg's ArchivesInflammatory Modulation of miR-155 Inhibits Doxorubicin?Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus?…[Pubmed:35715546]
AntioxidantsTherapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model?…[Pubmed:35204140]
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?…[]
Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation?…[]