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血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Platelet Factor 4 (PF4)

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

  • 血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)實(shí)驗(yàn)結(jié)果圖
  • SEA172Ra.jpg標(biāo)準(zhǔn)曲線圖
  • Certificate通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)血小板因子4(PF4)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的血小板因子4(PF4)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)93-10197
EDTA plasma(n=5)84-10198
heparin plasma(n=5)89-10397

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血小板因子4(PF4)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中血小板因子4(PF4)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)92-105%98-105%82-101%81-90%
EDTA plasma(n=5)88-96%98-105%82-102%85-94%
heparin plasma(n=5)93-101%95-102%80-98%85-94%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將血小板因子4(PF4)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血小板因子4(PF4)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血小板因子4(PF4)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血小板因子4(PF4)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。

相關(guān)產(chǎn)品

編號(hào)適用物種：Rattus norvegicus (Rat，大鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA172Ra02血小板因子4(PF4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA172Ra01血小板因子4(PF4)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA172Ra01血小板因子4(PF4)多克隆抗體WB; IHC; ICC; IP.
PAA172Ra02血小板因子4(PF4)多克隆抗體WB; IHC; ICC; IP.
LAA172Ra71血小板因子4(PF4)多克隆抗體(生物素標(biāo)記)WB; IHC; ICC.
LAA172Ra81血小板因子4(PF4)多克隆抗體(異硫氰酸熒光素標(biāo)記)WB; IHC; ICC; IF.
MAA172Ra21血小板因子4(PF4)單克隆抗體WB; IHC; ICC; IP.
SEA172Ra血小板因子4(PF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA172Ra血小板因子4(PF4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA172Ra01血小板因子4(PF4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志參考文獻(xiàn)
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Basic Research in CardiologySecretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilation[PubMed: 22899170]
PLoS OnePlatelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote Neuroinflammation[PubMed: PMC3608633]
Diving and Hyperbaric MedicineEffect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompression[Pubmed:25311322]
Journal of Functional FoodsA?Phellinus baumii–based supplement containing?Salvia miltiorrhiza?Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro?and?in vivo[science:S1756464616300676]
Plos oneAspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injury[pubmed:27583400]
international journal of molecular sciences Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.[pubmed:28272338]
Experimental?and?Therapeutic?MedicineFeasibility of improving platelet?rich plasma therapy by using chitosan with high platelet activation ability[pubmed:28450960]
Reproductive ToxicologyAspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in rats[Pubmed: 30528429]
INTERNATIONAL JOURNAL OF CARDIOLOGYShort and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablation[Pubmed: 30857843]
Available at SSRN 3335365?Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers[]
journal of biotechnologyNewly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes Hemostasis[Pubmed: 30927490]
Data in BriefData for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation[]
journal of thoracic and cardiovascular surgeryEvaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers[Pubmed: 32812629]
Marine DrugsThe Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents[Pubmed: 31533230]
Thrombosis ResearchFeasibility study of use of rabbit blood to evaluate platelet activation by medical devices[Pubmed: 31838449]
Nat CommunThe C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets[34099640]
VaccineThe effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female rats[Pubmed:35183388]