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基質(zhì)細(xì)胞衍生因子1(SDF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)

CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12

  • 基質(zhì)細(xì)胞衍生因子1(SDF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 基質(zhì)細(xì)胞衍生因子1(SDF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)產(chǎn)品包裝(模擬)
  • 基質(zhì)細(xì)胞衍生因子1(SDF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)實(shí)驗(yàn)結(jié)果圖
  • SEA122Po.jpg標(biāo)準(zhǔn)曲線圖
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)基質(zhì)細(xì)胞衍生因子1(SDF1),經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè),因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的基質(zhì)細(xì)胞衍生因子1(SDF1)(加標(biāo)樣品),重復(fù)測(cè)定并計(jì)算其均值,回收率為測(cè)定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)80-9087
EDTA plasma(n=5)78-9995
heparin plasma(n=5)90-10494

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次,分別計(jì)算不同濃度樣本的平均值及SD值。
批間差:選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定,每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次,分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)細(xì)胞衍生因子1(SDF1),并倍比稀釋成1:2,1:4,1:8,1:16的待測(cè)樣本,線性范圍即為稀釋后樣本中基質(zhì)細(xì)胞衍生因子1(SDF1)含量的測(cè)定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)98-105%91-101%78-91%95-104%
EDTA plasma(n=5)79-101%78-94%82-89%88-98%
heparin plasma(n=5)88-101%78-94%94-105%78-96%

穩(wěn)定性

經(jīng)測(cè)定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響,實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致,尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時(shí);
3. 吸棄,加檢測(cè)溶液A100µL,37°C孵育1小時(shí);
4. 洗板3次;
5. 加檢測(cè)溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實(shí)驗(yàn)原理

將基質(zhì)細(xì)胞衍生因子1(SDF1)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的基質(zhì)細(xì)胞衍生因子1(SDF1)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的基質(zhì)細(xì)胞衍生因子1(SDF1)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的基質(zhì)細(xì)胞衍生因子1(SDF1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測(cè)定吸光度(O.D.值),計(jì)算樣品濃度。

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RPA122Po01基質(zhì)細(xì)胞衍生因子1(SDF1)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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參考文獻(xiàn)

雜志參考文獻(xiàn)
Critical CareEndothelial progenitor cells (EPC) in sepsis with acute renal dysfunction (ARD)[Goescholar: source]
22The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels[ScienceDirect: S0142961209012654]
22The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms[ScienceDirect: S0142961211011422]
PhytomedicineTanshinone IIA increases recruitment of bone marrow mesenchymal stem cells to infarct region via up-regulating stromal cell-derived factor-1/CXC chemokine receptor 4 axis in a myocardial ischemia model[ScienceDirect: S0944711310003260]
Graefe's Archive for Clinical and Experimental OphthalmologyMonocyte/macrophages promote vasculogenesis in choroidal neovascularization in mice by stimulating SDF-1 expression in RPE cells[PubMed: 21655971]
Molecular TherapyPleiotropic beneficial effects of sonic hedgehog gene therapy in an experimental model of peripheral limb ischemia[PubMed: 21224834]
OncogeneElevated PLGF contributes to small-cell lung cancer brain metastasis[PubMed: 22797069]
International Journal of Nephrology Impairment and Differential Expression of PR3 and MPO on Peripheral Myelomonocytic Cells with Endothelial Properties in Granulomatosis with Polyangiitis[PubMed: 22792461]
Rheumatology InternationalSerum levels of selected chemokines in systemic lupus erythematosus patients[Pubmed: 22461186]
Experimental Eye ResearchHyperglycemia promotes vasculogenesis in choroidal neovascularization in diabetic mice by stimulating VEGF and SDF-1 expression in retinal pigment epithelial cells[Pubmed: 24780853]
AtherosclerosisThe non-alcoholic fraction of beer increases stromal cell derived factor 1 and the number of circulating endothelial progenitor cells in high cardiovascular risk subjects: A randomized clinical trial[Pubmed:24530788]
International Journal of CardiologyHypoxic exercise training improves cardiac/muscular hemodynamics and is associated with modulated circulating progenitor cells in sedentary men[Pubmed:24286591]
Tissue Engineering Part ATissue Inhibitor of Metalloproteinase-1 and -3 Improves Cardiac Function in an Ischemic Cardiomyopathy Model Rat[Pubmed:24814095]
Pharmacol Rep.?Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression[Pubmed:25150002]
Journal of Stroke and Cerebrovascular DiseasesCirculating CD133+ CD34+ Progenitor Cells and Plasma Stromal-Derived Factor-1Alpha: Predictive Role in Ischemic Stroke Patients[Pubmed:25444027]
Pharmacological ReportsThe MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders[Pubmed:25560580]
In Vitro Cellular &amp; Developmental Biology - AnimalStromal cell-derived factor 1 (SDF-1) accelerated skin wound healing by promoting the migration and proliferation of epidermal stem cells[Pubmed:25636237]
Pharmacological ReportsSerum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder[PubMed: 26481549]
Biochem Biophys Res CommunStromal cell-derived factor 1 promoted migration of adipose-derived stem cells to the wounded area in traumatic rats[PubMed: 26392311]
Am J Transl ResRole of SDF-1 and Wnt signaling pathway in the myocardial fibrosis of hypertensive rats[PubMed: 26396666]
Medicine (Baltimore)A Novel Combination of Calprotectin and CXCL13 for Predicting Malignancy in Patients with Exudative Pleural Effusion[PubMed: 26632727]
Pharmacological ReportsA study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity[Pubmed:27208878]
Frontiers in PsychiatryPlasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.[pubmed:28149283]
European?Journal?of?Applied?PhysiologyHigh-intensity?Interval?training?enhances?mobilization/functionality?of?endothelial?progenitor?cellsand?depressed?shedding?of?vascular?endothelial?cells?undergoing hypoxia.[pubmed:27761657]
Biochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseAMD3100 treatment attenuates pulmonary angiogenesis by reducing the c-kit (+) cells and its pro-angiogenic activity in CBDL rat lungs[pubmed:29233725]
International Journal of?Clinical?and Experimental PathologyPlatelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens[ISSN:1936-2625/IJCEP0058424]
BioMed Research International. c-Kit-Positive Adipose Tissue-Derived Mesenchymal Stem Cells Promote the Growth and Angiogenesis of Breast Cancer[pubmed:28573141]
Scientific Reports Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms.[pubmed:28842629]
BiomaterialsEffects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study.[pubmed:29017079]
Biomaterials?ScienceThe effects of Sr-incorporated micro/nano rough titanium surface on rBMSC migration and osteogenic differentiation for rapid osteointegration[Pubmed:29850672]
International Journal of Molecular?MedicineLow?intensity pulsed ultrasound promotes periodontal ligament stem cell migration through TWIST1?mediated SDF?1 expression[Pubmed:29620151]
Cardiovascular Drugs and Therapyβ‐blockers interfere with cell homing receptors and regulatory proteins in a model of spontaneously hypertensive rats[Pubmed:29752864]
CytotherapyMesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantation[Doi: 10.1016/j.jcyt.2018.07.007]
ACS?Applied?Materials?&?InterfacesFunctionalization of Silk Fibroin Electrospun Scaffolds via BMSC Affinity Peptide Grafting through Oxidative Self-Polymerization of Dopamine for Bone Regeneration[Pubmed: 30777748]
World?Journal of?Stem?CellsStromal cell-derived factor-1α promotes recruitment and differentiation of nucleus pulposus-derived stem cells[Pubmed: 30949297]
Stem Cells InternationalEffects of Stromal Cell-Derived Factor-1α Secreted in Degenerative Intervertebral Disc on Activation and Recruitment of Nucleus Pulposus-Derived Stem Cells[Pubmed: 31827537]
Stem Cell Res TherShort-wave enhances mesenchymal stem cell recruitment in fracture healing by increasing HIF-1 in callus[Pubmed: 32894200]
Journal of tissue engineering and regenerative medicineEnhanced recruitment and hematopoietic reconstitution of bone marrow‐derived mesenchymal stem cells in bone marrow failure by the SDF‐1/CXCR4[Pubmed: 32633015]
Acta BiomaterHighly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia[33878473]
pharmaceuticalsbFGF and SDF-1|á Improve In Vivo Performance of VEGF-Incorporating Small-Diameter Vascular Grafts[33800631]
Microb BiotechnolCombination of an engineered Lactococcus?lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice[34310856]