表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)真核蛋白
Eukaryotic Surfactant Associated Protein D (SPD)
SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D
- 編號EPB039Hu61
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源真核表達
- 宿主293F cell
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量37.1kDa
- 實際分子量44kDa(差異分析請參閱說明書)
- 片段與標簽Ala21~Phe375 with N-terminal His Tag
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 95%
- 等電點6.5
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
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參考文獻
| 雜志 | 參考文獻 |
| The American Journal of Rhinology & Allergy | Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response[Ingenta: art00005] |
| J Occup Health | Evaluation of acute oxidative stress induced by NiO nanoparticles in vivo and in vitro[PubMed: 21233593] |
| Inhalation Toxicology | Comparison of acute oxidative stress on rat lung induced by nano and fine-scale, soluble and insoluble metal oxide particles: NiO and TiO2[PubMed: 22642288] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps[PubMed: 23406594] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins[PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS[PubMed: PMC3787032] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge[Opus4:Source] | |
| PLoS One | The?Cerebral?Surfactant?System?and Its?Alteration?in?HydrocephalicConditions.[pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations?of?Ventricular?Enlargement?with?Rheologically?Active?SurfactantProteins?in?Cerebrospinal?Fluid.[pubmed:28101052] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.[pubmed:29167126] |
| Molecular Neurobiology | Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF?…[Doi: 10.1007/s12035-019-01648-z] |
| Respir Res | Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital?…[Pubmed:35189887] |
| Virology Journal | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1?…[Pubmed:35361235] |