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YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Peptide YY (PYY) ,etc. by FLIA (Flow Luminescence Immunoassay)

PYY1; Peptide tyrosine tyrosine

(注:單次混測多因子不超過8個指標 )

  • YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)84-9589
EDTA plasma(n=5)96-105101
heparin plasma(n=5)81-8985

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內加入適量的YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)99-105%78-103%82-91%87-94%
EDTA plasma(n=5)84-95%78-94%87-94%93-105%
heparin plasma(n=5)79-98%88-102%94-102%78-101%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠、檢測溶液A)標準品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
    37°C酶標板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠、標記VEGFA,標記的YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合,然后將未結合物洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的YY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

相關產(chǎn)品

編號適用物種:Canis familiaris; Canine (Dog,犬)應用(僅供研究使用,不用于臨床診斷!)
CPB067Ca21YY肽(PYY)卵白蛋白偶聯(lián)物Immunogen; SDS-PAGE; WB.
CEB067CaYY肽(PYY)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB067CaYY肽(PYY)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
International Journal of Sport StudiesThe Effects of Concurrent Resistance and Endurance Exercise on Hunger Feelings and PYY in Obese Men[Com:Source]
Annals of Applied Sport ScienceEffect of an Acute Incremental Exercise on Plasma Peptide YY, Neuropeptide Y and IGF-1 Concentrations in Young Athletes[Aassjournal:Source]
Biomedical ReportsCalprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents.[pubmed:28413639]
Acta DiabetologicaEffect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes[pubmed:28620678]
Biomedical ReportsCalprotectin in serum and zonulin in serum and feces are elevated after introduction of a diet with lower carbohydrate content and higher fiber, fat and protein contents[10.3892/br.2017.865]
Journal of Diabetes & MetabolismHigh Fiber Fat and Protein Contents Lead to Increased Satiety Reduced Sweet Cravings and Decreased Gastrointestinal Symptoms Independently of Anthropometric Hormonal and Metabolic Factors[10.4172/2155-6156.1000733?]
Frontiers in PhysiologyIleal Transposition Surgery Decreases Fat Mass and Improves Glucose Metabolism in Diabetic GK Rats: Possible Involvement of FGF21[Pubmed:29593555]
Food?and Nutrition?ResearchAlignments of endocrine, anthropometric, and metabolic parameters in type 2 diabetes after intervention with an Okinawa-based Nordic diet[Pubmed:29599686]
The Journal of NutritionGrape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs[Pubmed: 31175811]
The Journal of Nutritional BiochemistryBile acid receptor TGR5 is critically involved in preference for dietary lipids and obesity[Pubmed: 31812910]
Arch ToxicolSerotonin involvement in okadaic acid-induced diarrhoea in vivo[34148100]
foodsAppetite and Satiety Effects of the Acute and Regular Consumption of Green Coffee Phenols and Green Coffee Phenol/Oat β-Glucan Nutraceuticals in Subjects with?…[34828792]