骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Bone Morphogenetic Protein 2 (BMP2) ,etc. by FLIA (Flow Luminescence Immunoassay)
BMP2A; BMP-2A; Hemochromatosis Modifier
（注：單次混測多因子不超過8個指標 ）
- 編號LMA013Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.98-1000pg/mL
- 靈敏度最小可檢測劑量小于等于0.327 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 85-102 | 93 |
| EDTA plasma(n=5) | 84-99 | 88 |
| heparin plasma(n=5) | 97-105 | 101 |
| sodium citrate plasma(n=5) | 83-96 | 86 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 79-97% | 84-103% | 87-101% |
| EDTA plasma(n=5) | 92-101% | 94-105% | 97-104% | 79-98% |
| heparin plasma(n=5) | 97-104% | 85-101% | 79-94% | 86-104% |
| sodium citrate plasma(n=5) | 89-101% | 80-101% | 92-99% | 88-99% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA013Hu01 | 骨成型蛋白2(BMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA013Hu02 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Hu01 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA013Hu03 | 骨成型蛋白2(BMP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA013Hu02 | 骨成型蛋白2(BMP2)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA013Hu02 | 骨成型蛋白2(BMP2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA013Hu01 | 骨成型蛋白2(BMP2)多克隆抗體 | WB |
| MAA013Hu22 | 骨成型蛋白2(BMP2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA013Hu | 骨成型蛋白2(BMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA013Hu | 骨成型蛋白2(BMP2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA013Hu | 骨成型蛋白2(BMP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA013Hu01 | 骨成型蛋白2(BMP2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
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| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Medical Journal of Chinese People's Liberation Army | Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion[Source] |
| PLOS ONE | PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling[Pubmed:25470749] |
| Eur J Pharmacol | Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy[PubMed: 25934570] |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy[PubMed: 25871861] |
| Journal of Cellular Biochemistry | Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway[PubMed: 26681207] |
| International Journal of Nanomedicine | Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis[Pubmed:27042064] |
| Research report | EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO[files:p013-022] |
| Gene? | Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE[Pubmed:27196063] |
| Journal of Materials Chemistry B | Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair[DOI:10.1039/C7TB00640C] |
| Scientific Reports | 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering.[pubmed:29042614] |
| Journal of Applied Biomaterials & Functional Materials | Demineralized dentin and enamel matrices as suitable substrates for bone regeneration[pubmed:28731486] |
| Journal of?Clinical?Periodontology? | Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats[Pubmed:29574908] |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats[Pubmed:29898457] |
| Journal of?Applied?Biomaterials?&?Functional?Materials | BMP-2 and type I collagen preservation in human deciduous teeth after demineralization[Pubmed:30045659] |
| Applied Surface Science | Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction[] |
| Materials Science and Engineering: C | Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration[] |
| Biomaterials Science | Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration[] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats[Pubmed: 32004919] |
| Journal of Industrial and Engineering Chemistry | Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration[] |
| BMC Musculoskeletal Disorders | Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse[] |
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| Tissue Eng Regen Med | Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro[34302695] |
| Toxicon | The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats[34742779] |
| ACS Biomater Sci Eng | Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum[34472855] |
| Phytomedicine | Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane[Pubmed:35278899] |