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促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Corticotropin Releasing Factor (CRF) ,etc. by FLIA (Flow Luminescence Immunoassay)

CRH; Corticotropin Releasing Hormone; Corticoliberin

(注:單次混測多因子不超過8個指標 )

  • 促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)93-10196
EDTA plasma(n=5)83-9690
heparin plasma(n=5)78-9486
sodium citrate plasma(n=5)96-105101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)88-97%78-89%86-96%78-97%
EDTA plasma(n=5)94-102%88-101%94-101%86-98%
heparin plasma(n=5)96-104%89-99%84-95%91-101%
sodium citrate plasma(n=5)93-101%90-103%81-93%87-103%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠、檢測溶液A)標準品或樣本50μL及磁珠10μL,加檢測溶液A50μL,
    37°C酶標板振蕩器孵育60分鐘;
3. 磁吸洗板3次;
4. 加檢測溶液B100μL,37°C振動孵育30分鐘;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠、標記VEGFA,標記的促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合,然后將未結(jié)合物洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關(guān)。

贈品

相關(guān)產(chǎn)品

編號適用物種:Sus scrofa; Porcine (Pig,豬)應(yīng)用(僅供研究使用,不用于臨床診斷!)
CEA835Po促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA835Po促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
Toxicology LettersRegulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats[Pubmed:24440345]
Bipolar?Disord.Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β?[Pubmed:24862833]
PeptidesDifferential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level[Pubmed:25236411]
Ann DermatolAcute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth.[pubmed:27746640]
NeuroscienceHeat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation[pubmed:28663093]
Biomed Research InternationalColonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation[pubmed:29082242]
Biochemical PharmacologyEffects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats[Pubmed:30028991]
Frontiers in PharmacologyVaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?…[Pubmed:30038568]
Toxicology LettersPerfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors[Pubmed:29807116]
Neuropsychiatric disease and treatmentThe depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?…[Pubmed:29379286]
BMC?Veterinary?ResearchHypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?…[Pubmed:29540178]
Bioscience ResearchBioscience Research[]
FASEB JournalContribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice[Pubmed: 31431066]
Aging CellAltered glucocorticoid metabolism represents a feature of macroph‐aging[Pubmed: 32463582]
Endocr JImpairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress[33408312]
CRYOBIOLOGYO-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress[33651993]