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防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Defensin Beta 2 (DEFb2) ,etc. by FLIA (Flow Luminescence Immunoassay)

B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1

（注：單次混測多因子不超過8個指標 ）

  • 防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • 防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)產品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)89-9893
EDTA plasma(n=5)79-10296
heparin plasma(n=5)94-10198
sodium citrate plasma(n=5)96-104101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)82-92%80-103%89-102%79-88%
EDTA plasma(n=5)94-102%96-104%79-101%88-99%
heparin plasma(n=5)93-101%81-89%93-101%85-101%
sodium citrate plasma(n=5)93-105%92-101%98-105%97-104%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合，然后加入生物素化的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產品

編號適用物種：Homo sapiens (Human，人)應用(僅供研究使用，不用于臨床診斷！)
APA072Hu01防御素β2(DEFb2)活性蛋白Cell?culture;?Activity?Assays.
RPA072Hu01防御素β2(DEFb2)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA072Hu01防御素β2(DEFb2)多克隆抗體WB
LAA072Hu71防御素β2(DEFb2)多克隆抗體(生物素標記)WB; IHC; ICC.
MAA072Hu22防御素β2(DEFb2)單克隆抗體WB; IHC; ICC; IP.
SEA072Hu防御素β2(DEFb2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA072Hu防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.
KSA072Hu01防御素β2(DEFb2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志參考文獻
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University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source]
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