防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Defensin Beta 2 (DEFb2) ,etc. by FLIA (Flow Luminescence Immunoassay)
B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMA072Hu
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍1.95-2000pg/mL
- 靈敏度最小可檢測劑量小于等于0.65 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 89-98 | 93 |
| EDTA plasma(n=5) | 79-102 | 96 |
| heparin plasma(n=5) | 94-101 | 98 |
| sodium citrate plasma(n=5) | 96-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-92% | 80-103% | 89-102% | 79-88% |
| EDTA plasma(n=5) | 94-102% | 96-104% | 79-101% | 88-99% |
| heparin plasma(n=5) | 93-101% | 81-89% | 93-101% | 85-101% |
| sodium citrate plasma(n=5) | 93-105% | 92-101% | 98-105% | 97-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA072Hu01 | 防御素β2(DEFb2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA072Hu01 | 防御素β2(DEFb2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA072Hu01 | 防御素β2(DEFb2)多克隆抗體 | WB |
| LAA072Hu71 | 防御素β2(DEFb2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA072Hu22 | 防御素β2(DEFb2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA072Hu | 防御素β2(DEFb2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA072Hu | 防御素β2(DEFb2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA072Hu01 | 防御素β2(DEFb2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Appl Environ Microbiol. | Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell Line[Asm: Source] |
| Plos Pathogens | HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in?Pseudomonas aeruginosa?Keratitis[PubMed: PMC3715414] |
| British Journal of Nutrition | Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han Chinese[Pubmed: 24124699] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis[Waikato:Source] |
| Vaccine | On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment[Pubmed:25444792] |
| International Journal of Dairy Technology | Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formula[Doi: 10.1111] |
| Molecular Nutrition Food Research | Green?tea?polyphenol?epigallocatechin-3-gallate?improves?epithelial?barrier?function?by?inducing?the?productionof?antimicrobial?peptide?pBD-1?and?pBD-2?in?monolayers?of?porcine?intestinal?epithelial?IPEC-J2?cells.[Pubmed:26991948] |
| Respiratory Research | Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner.[pubmed:27655266] |
| Key Engineering Materials | The Level of Inflammatory Cytokines and Antimicrobial Peptides after Composite Material Implantation and Contamination with Bacterial Culture.[16629795] |
| Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique[Pubmed:29374283] |
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| Journal of Dermatological?Science | Glyoxal-induced exacerbation of pruritus and dermatitis is associated with staphylococcus aureus colonization in the skin of a rat model of atopic dermatitis[Pubmed:29496360] |
| International Immunopharmacology | Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase?…[Pubmed: 30342347] |
| 拉脫維亞科學(xué)院院刊 | Influence of Antibiotic-Impregnated Biomaterials on Inflammatory Cytokines[] |
| Veterinary?Research | Bovine fetal mesenchymal stem cells exert antiproliferative effect against mastitis causing pathogen Staphylococcus aureus[Pubmed: 30975214] |
| Journal of Immunology Research | Damage-Associated Molecular Patterns and Myeloid-Derived Suppressor Cells in Bronchoalveolar Lavage Fluid in Chronic Obstructive Pulmonary Disease Patients[] |
| Veterinary Research | Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection[Pubmed: 31221216] |
| Animals | Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport[33375218] |
| Sci Rep | Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus[34362937] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?…[Pubmed:35687771] |