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吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Indoleamine-2,3-Dioxygenase (IDO) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD107B; INDO; Indoleamine-Pyrrole 2,3 Dioxygenase

（注：單次混測多因子不超過8個指標 ）

  • 吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)78-9981
EDTA plasma(n=5)92-10198
heparin plasma(n=5)93-10196

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)78-105%96-103%82-96%88-101%
EDTA plasma(n=5)91-105%84-104%89-97%81-101%
heparin plasma(n=5)97-104%82-90%94-103%78-89%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號適用物種：Mus musculus (Mouse，小鼠)應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB547Mu01吲哚胺2,3-雙加氧酶(IDO)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPB547Mu02吲哚胺2,3-雙加氧酶(IDO)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB547Mu02吲哚胺2,3-雙加氧酶(IDO)多克隆抗體WB; IHC; ICC; IP.
PAB547Mu01吲哚胺2,3-雙加氧酶(IDO)多克隆抗體WB; IHC; ICC; IP.
SEB547Mu吲哚胺2,3-雙加氧酶(IDO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB547Mu吲哚胺2,3-雙加氧酶(IDO)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
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Clinical TransplantationPotential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT[Wiley: source]
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