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血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Thrombomodulin (TM) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD141; THBD; BDCA3; THRM; Fetomodulin

（注：單次混測多因子不超過8個指標 ）

  • 血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • 血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)產(chǎn)品包裝(模擬)
  • Certificate通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本回收率范圍(%)平均回收率(%)
serum(n=5)80-9980
EDTA plasma(n=5)86-10291
heparin plasma(n=5)92-10196
sodium citrate plasma(n=5)91-9996

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本1:21:41:81:16
serum(n=5)91-101%85-103%81-91%78-104%
EDTA plasma(n=5)80-90%95-105%84-101%89-103%
heparin plasma(n=5)89-96%78-101%83-93%93-105%
sodium citrate plasma(n=5)78-99%91-101%83-92%91-99%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
    37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。

贈品

相關產(chǎn)品

編號適用物種：Homo sapiens (Human，人)應用(僅供研究使用，不用于臨床診斷！)
APA529Hu61血栓調(diào)節(jié)蛋白(TM)活性蛋白Cell?culture;?Activity?Assays.
RPA529Hu03血栓調(diào)節(jié)蛋白(TM)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA529Hu02血栓調(diào)節(jié)蛋白(TM)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA529Hu62血栓調(diào)節(jié)蛋白(TM)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA529Hu01血栓調(diào)節(jié)蛋白(TM)重組蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA529Hu01血栓調(diào)節(jié)蛋白(TM)多克隆抗體WB;ICC/IF
PAA529Hu02血栓調(diào)節(jié)蛋白(TM)多克隆抗體WB; IHC
PAA529Hu03血栓調(diào)節(jié)蛋白(TM)多克隆抗體ICC/IF
LAA529Hu71血栓調(diào)節(jié)蛋白(TM)多克隆抗體(生物素標記)WB; IHC; ICC.
MAA529Hu22血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB; IHC; ICC; IP.
MAA529Hu26血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB; IHC; ICC; IP.
MAA529Hu25血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB; IHC; ICC; IP.
MAA529Hu27血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB; IHC; ICC; IP.
MAA529Hu28血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB
MAA529Hu29血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB
MAA529Hu21血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB; IHC
RAA529Hu21血栓調(diào)節(jié)蛋白(TM)重組抗體WB; IFC; IHC; IP.
MAA529Hu23血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB
MAA529Hu24血栓調(diào)節(jié)蛋白(TM)單克隆抗體WB
FAA529Hu52抗血栓調(diào)節(jié)蛋白(TM)單克隆抗體(別藻藍蛋白標記)FCM
SEA529Hu血栓調(diào)節(jié)蛋白(TM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA529Hu血栓調(diào)節(jié)蛋白(TM)等多因子檢測試劑盒(流式熒光發(fā)光法)FLIA Kit for Antigen Detection.

參考文獻

雜志參考文獻
PLoS OneA Non-Lethal Traumatic/Hemorrhagic Insult Strongly Modulates the Compartment-Specific PAI-1 Response in the Subsequent Polymicrobial Sepsis[PubMed: PMC3568129]
Clin Appl Thromb Hemost.Is There Any Association Between Thrombosis and Tissue Factor Pathway Inhibitor Levels in Patients With Vitamin D Deficiency?[Pubmed: 24203351]
медицинские наукиКЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В …[Ru:Source]
FASEB J.Endothelial mineralocorticoid receptor activation enhances endothelial protein C receptor and decreases vascular thrombosis in mice[Pubmed:24451386]
Gynecol Endocrinol.The effect of hormone therapy on biochemical and ultrasound parameters associated with atherosclerosis in 46, XY DSD individuals with female phenotype[Pubmed:24911331]
Informa HealthcareChanges in cardiac thrombomodulin and heat shock transcription factor 1 expression and peripheral thrombomodulin and catecholamines during hypothermia in rats[Pubmed:25109347]
Bone Marrow Transplant.Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen[Pubmed:25243628]
Biomed Res IntBasic Fibroblast Growth Factor Ameliorates Endothelial Dysfunction in Radiation-Induced Bladder Injury[PubMed: 26351640]
Molecular Nutrition & Food ResearchTrans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors[PubMed: 25546502]
Acta BiomaterAntioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study[PubMed: 26689470]
MEDICINE AND EDUCATION IN SIBERIAКЛЕТОЧНО-МОЛЕКУЛЯРНЫЕ МАРКЕРЫ ЭНДОТЕЛИАЛЬНОЙ ДИСФУНКЦИИ И СИСТЕМНОГО ГЕМОСТАЗА ПРИ ВИБРАЦИОННОЙ БОЛЕЗНИ В СОЧЕТАНИИ С АРТЕРИАЛЬНОЙ ГИПЕРТЕНЗИЕЙ[Cozo: Mos]
Acta BiomaterialiaAntioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study[Pubmed:26689470]
Bone Marrow TransplantationThe?fifth?epidermal?growth?factor-like?region?of?thrombomodulin?exerts?cytoprotective?functionand?prevents?SOS?in a?murine?model.[pubmed:27427915]
BMC Vet Res.Blood markers of fibrinolysis and endothelial activation in canine babesiosis.[pubmed:28363279]
HaematologicaCytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain[Pubmed:29903766]
Biomedicine & PharmacotherapyPuerarin prevents vascular endothelial injury through suppression of NF-κB activation in LPS-challenged human umbilical vein endothelial cells[Pubmed:29775893]
Scientific ReportsAssociation between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea[Pubmed: 31882827]
Intensive Care Medicine ExperimentalPreservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental?…[34169407]