中期因子(MK)重組蛋白
Recombinant Midkine (MK)
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
- 編號RPA631Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量20.0kDa
- 實際分子量20kDa(差異分析請參閱說明書)
- 片段與標簽Ala22~Asp143 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點9.4
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 1008¥ 2520¥ 5040¥ 15120¥ 37800
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
SDS-PAGE圖
通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA631Hu02 | 中期因子(MK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA631Hu01 | 中期因子(MK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA631Hu01 | 中期因子(MK)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA631Hu02 | 中期因子(MK)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA631Hu01 | 中期因子(MK)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA631Hu71 | 中期因子(MK)多克隆抗體(生物素標記) | WB |
| LAA631Hu81 | 中期因子(MK)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA631Hu22 | 中期因子(MK)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA631Hu21 | 中期因子(MK)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA631Hu | 中期因子(MK)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA631Hu | 中期因子(MK)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA631Hu01 | 中期因子(MK)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin[Springer: Source] |
| 33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and …[10.1016:j.ebiom.2017.11.020] |
| Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice[pubmed:28781288] |
| EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and?…[Pubmed:29233575] |
| Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall?…[Pubmed:29797475] |
| Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese[:] | |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study[Pubmed: 32198440] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells[Pubmed: 33206337] |