Ⅰ型膠原α2(COL1a2)重組蛋白
Recombinant Collagen Type I Alpha 2 (COL1a2)
COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain
- 編號RPA215Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量33.2kDa
- 實際分子量33kDa(差異分析請參閱說明書)
- 片段與標簽Val1109~Lys1372 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點5.8
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
- 價格¥ 1080¥ 2700¥ 5400¥ 16200¥ 40500
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA215Ra01 | Ⅰ型膠原α2(COL1a2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA215Ra01 | Ⅰ型膠原α2(COL1a2)活性蛋白 | Cell?culture;?Activity?Assays. |
| LAA215Ra71 | Ⅰ型膠原α2(COL1a2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA215Ra | Ⅰ型膠原α2(COL1a2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA215Ra | Ⅰ型膠原α2(COL1a2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Plos one | Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine[PubMed: PMC3581499] |
| J Proteomics | Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)[PubMed: 26718731] |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis[pubmed:28665955] |
| American Journal of Translational Research | A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model[Pubmed:29511441] |
| Sci Rep | The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs)[Pubmed: 33208768] |
| Experimental and Therapeutic Medicine | Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice[33986847] |