表面活性物質(zhì)關(guān)聯(lián)蛋白A(SFTPA1)重組蛋白
Recombinant Pulmonary Surfactant Associated Protein A1 (SFTPA1)
PRL; SFTPA; PSAP; PSPA; SFTP; SPA; COLEC4; PSP-A; SFTP1; SFTPA1B; SP-A1; Collectin-4; Alveolar proteinosis protein; 35 kDa pulmonary surfactant-associated protein; Surfactant Associated Protein A
- 編號RPA890Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位細胞外基質(zhì)
- 預測分子量44.6kDa
- 實際分子量44kDa(差異分析請參閱說明書)
- 片段與標簽Glu21~Phe248 with His and TRxA Tag
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點4.6
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質(zhì)量體系認證
序列
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
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參考文獻
| 雜志 | 參考文獻 |
| The American Journal of Rhinology & Allergy | Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response[Ingenta: art00005] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps[PubMed: 23406594] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins[PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS[PubMed: PMC3787032] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge[Opus4:Source] | |
| Mol Med Rep | Expression of surfactant protein-A in exhaled breath condensate of patients with chronic obstructive pulmonary disease[PubMed: 26707652] |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)[Pubmed:27337142] |
| PLoS One | The?Cerebral?Surfactant?System?and Its?Alteration?in?HydrocephalicConditions.[pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations?of?Ventricular?Enlargement?with?Rheologically?Active?SurfactantProteins?in?Cerebrospinal?Fluid.[pubmed:28101052] |
| Frontiers in?Aging?Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid[PMC5209370] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.[pubmed:29167126] |
| Molecular biosystems | Metabolic profiling of asthma in mice and the interventional effects of SPA using liquid chromatography and Q-TOF mass spectrometry[pubmed:28463380] |
| PLoS One | Evaluation of surfactant proteins A, B, C, and D in articular cartilage, synovial membrane and synovial fluid of healthy as well as patients with osteoarthritis and?…[Pubmed: 30235245] |
| PLOS ONE | Organic barn dust inhibits surfactant protein D production through protein kinase-c alpha dependent increase of GPR116[Pubmed: 30543664] |
| Molecular Neurobiology | Elevated levels of multifunctional surfactant proteins in cerebrospinal fluid are associated with signs of increased cerebrospinal fluid flow in cranial magnetic?…[Doi: 10.1007/s12035-017-0835-5] |
| Molecular Neurobiology | Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF?…[Doi: 10.1007/s12035-019-01648-z] |
| Antioxidants (Basel) | Oxidative Stress and Inflammatory Mediators in Exhaled Breath Condensate of Patients with Pulmonary Tuberculosis. A Pilot Study with a Biomarker Perspective[34679707] |