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抗核纖層蛋白B1(LMNB1)單克隆抗體

編號(hào)CAF548Hu22
物種Homo sapiens (Human，人)相同的名稱，不同的物種。
純化方式蛋白A + 蛋白G親和純化
標(biāo)記物無標(biāo)記物
緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性狀液體
濃度1mg/mL
且適物種Cavia (Guinea pig ，豚鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊)
應(yīng)用Loading Control of WB
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規(guī)格20μl100μl200μl1ml10ml
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10ug/ml Mouse Anti-Human TUBb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Ovarian cancer Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human amygdalitis Tissue;
Primary Ab: 10ug/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human LMNB1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant LMNB1, Human.
Western Blot analysis of LMNB1 in various cell lines
Lane1: DU145 whole cell lysate
Lane2: HL60 whole cell lysate
Lane3: THP-1 whole cell lysate
Lane4: HEC-1-B whole cell lysate
Lysates/proteins at 20μg per lane.
Primary Ab: anti-LMNB1 antibody (CAF548Hu22) at 0.1ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique
Calculated MW: 66KD
Observed MW: 66KD
Exposure time: 1 min
Western Blot; Sample: Lane1: Bovine Lung lysate; Lane2: Bovine Kidney lysate; Lane3: Caprine Lung lysate; Lane4: Caprine Kidney lysate; Lane5: Canine Kidney lysate; Lane6: Rabbit Kidney lysate; Lane7: Human Lung lysate; Lane8: 293T cell lysate; Lane9: Porcine Lung lysate; Lane10: Porcine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Human LMNB1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

該抗體是針對(duì)LMNB1的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別LMNB1。

Western blotting: 0.001-1ug/ml
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

雜志	參考文獻(xiàn)
Journal of Biological Chemistry	Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids[Pubmed: 30237167]

編號(hào)	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPF548Hu01	核纖層蛋白B1(LMNB1)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAF548Hu01	核纖層蛋白B1(LMNB1)多克隆抗體	WB; IHC; ICC; IP.
CAF548Hu01	抗核纖層蛋白B1(LMNB1)多克隆抗體	Loading Control of WB
MAF548Hu27	核纖層蛋白B1(LMNB1)單克隆抗體	WB; IHC; ICC; IP.
MAF548Hu22	核纖層蛋白B1(LMNB1)單克隆抗體	WB; ICC/IF
MAF548Hu23	核纖層蛋白B1(LMNB1)單克隆抗體	WB
MAF548Hu24	核纖層蛋白B1(LMNB1)單克隆抗體	WB; IHC; ICC; IP.
MAF548Hu25	核纖層蛋白B1(LMNB1)單克隆抗體	WB; IHC; ICC; IP.
MAF548Hu26	核纖層蛋白B1(LMNB1)單克隆抗體	WB
MAF548Hu28	核纖層蛋白B1(LMNB1)單克隆抗體	WB; IHC; ICC; IP.
MAF548Hu21	核纖層蛋白B1(LMNB1)單克隆抗體	Loading Control of WB; IHC; ICC; IP.
CAF548Hu22	抗核纖層蛋白B1(LMNB1)單克隆抗體	Loading Control of WB