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胱天蛋白酶14(CASP14)多克隆抗體

編號(hào)PAA457Mu01
物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
來源多抗制備
宿主兔
效價(jià)-
Ig類型IgG
純化方式抗原特異性親和純化
標(biāo)記物無標(biāo)記物
免疫原RPA457Mu01-胱天蛋白酶14(CASP14)重組蛋白
緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性狀液體
濃度500μg/mL
且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。
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DAB staining on IHC-P; Samples: Mouse Skin Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse CASP14 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant CASP14, Mouse.
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Skin lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Mouse CASP14 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Cerebrum lysate; Lane3: MCF7 cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.5μg/ml Rabbit Anti-Mouse CASP14 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CASP14 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 1μg/ml Rabbit Anti-Mouse CASP14 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Mouse Skin lysate; Lane2: Rat Heart lysate; Lane3: Rat Cerebrum lysate
Primary Ab: 0.5μg/ml Rabbit Anti-Mouse CASP14 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

該抗體是針對(duì)CASP14的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CASP14。

免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學(xué)：5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué)：5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。

雜志	參考文獻(xiàn)
Journal of Dermatological Science	Pyrrolidone carboxylic acid levels or caspase-14 expression in the corneocytes of lesional skin correlates with clinical severity, skin barrier function and lesional inflammation in atopic dermatitis[Pubmed:25315296]
In Vitro Cell Dev Biol Anim	Luteolin induces caspase-14-mediated terminal differentiation in human epidermal keratinocytes[PubMed: 26427706]
Applied?Biochemistry?and?Biotechnology	Enhanced Induction of Apoptosis in HaCaT Cells by Luteolin Encapsulated in PEGylated Liposomes—Role of Caspase-3/Caspase-14[Pubmed: 30390204]

編號(hào)	適用物種：Mus musculus (Mouse，小鼠)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA457Mu01	胱天蛋白酶14(CASP14)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA457Mu02	胱天蛋白酶14(CASP14)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA457Mu02	胱天蛋白酶14(CASP14)多克隆抗體	WB; IHC; ICC; IP.
PAA457Mu01	胱天蛋白酶14(CASP14)多克隆抗體	WB; IHC; ICC; IP.
SEA457Mu	胱天蛋白酶14(CASP14)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMA457Mu	胱天蛋白酶14(CASP14)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.
KSA457Mu01	胱天蛋白酶14(CASP14)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)	Main materials for "Do It (ELISA Kit) Yourself".