半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體
Polyclonal Antibody to Cystatin B (CSTB)
Cys-B; CST6; EPM1; PME; STFB; CPI-B; Stefin B; Liver thiol proteinase inhibitor
- 編號PAJ322Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPJ322Hu01-半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應(yīng)用WB;IHC;ICC/IF;FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Thyroid Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CSTB Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Esophagus cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human CSTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 10μg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 1.3μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Figure. Western Blot; Sample: Recombinant CSTB, Human.
Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: CSTB knockout HepG2 cell lysate; <br/>Predicted MW: 11kDa <br/>Observed MW: 14kDa<br/>Primary Ab: 2μg/ml Rabbit Anti-Human CSTB Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human Liver lysate; <br/>Primary Ab: 2μg/ml Rabbit Anti-Human CSTB Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: A549 cell lysate;
Primary Ab: 0.05μg/ml Rabbit Anti-Human CSTB Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human A431 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CSTB Polyclonal Antibody (Catalog PAJ322Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CSTB的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CSTB。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
For flow cytometry: 0.5-2 μg per 10^6 cells in 100 μl volume.
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ322Hu01 | 半胱氨酸蛋白酶抑制劑B(CSTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ322Hu01 | 半胱氨酸蛋白酶抑制劑B(CSTB)多克隆抗體 | WB;IHC;ICC/IF;FCM |
| MAJ322Hu21 | 半胱氨酸蛋白酶抑制劑B(CSTB)單克隆抗體 | WB; IHC; ICC; IP. |
| SEJ322Hu | 半胱氨酸蛋白酶抑制劑B(CSTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ322Hu | 半胱氨酸蛋白酶抑制劑B(CSTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| AIDS. | Cathepsin B and cystatin B in HIV-seropositive women are associated with infection and HIV-1-associated neurocognitive disorders[PubMed: 23291538] |
| International Journal of Circumpolar Health | Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer[PubMed: PMC3754495] |
| PLoS One. | Dysregulation of Macrophage-Secreted Cathepsin B Contributes to HIV-1-Linked Neuronal Apoptosis[PubMed: PMC3365072] |
| Journal of Neuroimmune Pharmacology | Cocaine potentiates cathepsin B secretion and neuronal apoptosis from HIV-infected macrophages[Pubmed:25209871] |
| European Journal of Cancer Supplements | A91: Endogenous inhibitors of cysteine proteases and preform of cathepsin B in cancer of reproductive system[Science: Article] |
| Annals of Clinical Biochemistry | Diagnostic specificity and sensitivity of PIVKAII, GP3, CSTB, SCCA1 and HGF for the diagnosis of hepatocellular carcinoma in patients with alcoholic liver cirrhosis.[pubmed:28766361] |