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干擾素調(diào)節(jié)因子3(IRF3)多克隆抗體

Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
AF488 staining on IF;
Sample: NIH/3T3 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: Rat Kidney lysate Primary Ab: 0.6μg/ml Rabbit Anti-Human IRF3 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 3μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Hela cell lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Human IRF3 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human IRF3 Polyclonal Antibody (Catalog PAB589Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

該抗體是針對IRF3的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別IRF3。

Western blotting: 0.01-2μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.

經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。

雜志	參考文獻
PLOS?Pathogens	RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production[pubmed:29186193]

編號	適用物種：Homo sapiens (Human，人)	應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB589Hu01	干擾素調(diào)節(jié)因子3(IRF3)重組蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAB589Hu01	干擾素調(diào)節(jié)因子3(IRF3)多克隆抗體	WB; ICC/IF; FCM
MAB589Hu22	干擾素調(diào)節(jié)因子3(IRF3)單克隆抗體	WB; IHC; ICC; IP.
SEB589Hu	干擾素調(diào)節(jié)因子3(IRF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB589Hu	干擾素調(diào)節(jié)因子3(IRF3)等多因子檢測試劑盒(流式熒光發(fā)光法)	FLIA Kit for Antigen Detection.