鞘脂激活蛋白原(PSAP)多克隆抗體
Polyclonal Antibody to Prosaposin (PSAP)
SAP1; GLBA; CSAct; Dispersin; Pro-Saposin; Proactivator polypeptide; Glucosylceramidase activator; Sulfatide/GM1 activator; Cerebroside sulfate activator; Sphingolipid Activator 1
- 編號(hào)PAC756Hu01
- 物種Homo sapiens (Human，人)相同的名稱(chēng)，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類(lèi)型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPC756Hu01-鞘脂激活蛋白原(PSAP)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC,ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Uterus Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Vector Red staining on IHC-P;
Samples: Human Spleen Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
DAB staining on IHC-P;
Sample: Porcine Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Cardiac Muscle Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human PSAP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
Figure. Western Blot ; Sample: Recombinant PSAP, Human.
Western Blot; Samples: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: A431 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: PSAP knockout 293T cell lysate;
Predicted MW: 58kd
Observed MW: 60kd
Primary Ab: 2μg/ml Rabbit Anti-Human PSAP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)PSAP的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別PSAP。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC756Hu01 | 鞘脂激活蛋白原(PSAP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC756Hu01 | 鞘脂激活蛋白原(PSAP)多克隆抗體 | WB,IHC,ICC/IF |
| MAC756Hu22 | 鞘脂激活蛋白原(PSAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC756Hu21 | 鞘脂激活蛋白原(PSAP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC756Hu23 | 鞘脂激活蛋白原(PSAP)單克隆抗體 | ICC/IF |
| MAC756Hu24 | 鞘脂激活蛋白原(PSAP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC756Hu | 鞘脂激活蛋白原(PSAP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC756Hu | 鞘脂激活蛋白原(PSAP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC756Hu01 | 鞘脂激活蛋白原(PSAP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Translational Research in Oral Oncology | Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer[Doi: 10.1177/2057178X18800534] |
| PeerJ | Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cycles[Pubmed: 31844578] |
| CANCER GENOMICS & PROTEOMICS | Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell Secretome[Pubmed: 32345664] |
| Frontiers in Microbiology | Surface Protein Dispersin of Enteroaggregative Escherichia coli Binds Plasminogen That Is Converted Into Active Plasmin[Pubmed: 32625178] |