70kDa熱休克蛋白5(HSPA5)單克隆抗體
Monoclonal Antibody to Heat Shock 70kDa Protein 5 (HSPA5)
MIF2; BIP; GRP78; Immunoglobulin heavy chain-binding protein; Glucose Regulated Protein 78; Binding Immunoglobulin Protein; Endoplasmic reticulum lumenal Ca binding grp78
- 編號(hào)MAC343Mu24
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C7
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原RPC343Mu01-70kDa熱休克蛋白5(HSPA5)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價(jià)格¥ 711¥ 1659¥ 2370¥ 5925¥ 23700
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Sample: Mouse Cerebrum Tissue; Primary Ab: 20μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Testis lysate; Lane3: A549 cell lysate Primary Ab: 2μg/ml Mouse Anti-Mouse HSPA5 Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)HSPA5的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HSPA5。
用法
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC343Mu02 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC343Mu01 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC343Mu61 | 70kDa熱休克蛋白5(HSPA5)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC343Mu03 | 70kDa熱休克蛋白5(HSPA5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC343Mu02 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC343Mu01 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體 | WB; IHC |
| LAC343Mu71 | 70kDa熱休克蛋白5(HSPA5)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC343Mu25 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC343Mu22 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC343Mu21 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC343Mu27 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC343Mu26 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | ICC/IF |
| MAC343Mu23 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC343Mu24 | 70kDa熱休克蛋白5(HSPA5)單克隆抗體 | WB; IHC; ICC; IP. |
| FAC343Mu02 | 抗70kDa熱休克蛋白5(HSPA5)單克隆抗體 | FCM |
| SEC343Mu | 70kDa熱休克蛋白5(HSPA5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC343Mu | 70kDa熱休克蛋白5(HSPA5)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC343Mu | 70kDa熱休克蛋白5(HSPA5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC343Mu01 | 70kDa熱休克蛋白5(HSPA5)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 41 | Deficiency of Rac1 Blocks NADPH Oxidase Activation, Inhibits Endoplasmic Reticulum Stress, and Reduces Myocardial Remodeling in a Mouse Model of Type 1 Diabetes.[PubMed: 20522592] |
| pharmacological research | Neuroprotection in non-transgenic and transgenic mouse models of Alzheimer's disease by positive modulation of σ1 receptors[Pubmed: 31048034] |
| EPILEPSY & BEHAVIOR | Fenfluramine acts as a positive modulator of sigma-1 receptors[Pubmed: 32169824] |
| Journal of Oncology | Using Serological Proteome Analysis to Identify and Evaluate Anti-GRP78 Autoantibody as Biomarker in the Detection of Gastric Cancer[33381181] |
| Heliyon | Glucose-regulated protein 78 (GRP78) in renal cell carcinoma: A novel biomarker for predicting tumor behavior[] |
| Medicine (Baltimore) | Levels of circulating GRP78 and CHOP in endoplasmic reticulum stress pathways in Chinese type 2 diabetic kidney disease patients[34414940] |