磷酸肌醇依賴性蛋白激酶1(PDPK1)多克隆抗體
Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)
PDK1; PkB-Like 1
- 編號PAC246Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原RPC246Hu01-磷酸肌醇依賴性蛋白激酶1(PDPK1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.35mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 630¥ 1470¥ 2100¥ 5250¥ 21000
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 30μg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 30μg/ml Rabbit Anti-Human PDPK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: MCF7 cell
Primary Ab: 20μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
Figure. Western Blot; Sample: Recombinant PDPK1, Human.
Western Blot; Sample: MCF7 cell lysate
Primary Ab: 0.5μg/ml Rabbit Anti-Human PDPK1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對PDPK1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別PDPK1。
用法
Western blotting: 0.01-2μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC246Hu01 | 磷酸肌醇依賴性蛋白激酶1(PDPK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC246Hu02 | 磷酸肌醇依賴性蛋白激酶1(PDPK1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC246Hu01 | 磷酸肌醇依賴性蛋白激酶1(PDPK1)多克隆抗體 | WB; IHC; ICC/IF |
| FAC246Hu01 | 抗磷酸肌醇依賴性蛋白激酶1(PDPK1)多克隆抗體 | Flow cytometry. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Oncotarget | Nucleoside reverse transcriptase inhibitor-induced rat oocyte dysfunction and low fertility mediated by autophagy[Pubmed:29423092] |