蛋白二硫化物異構(gòu)酶A4(PDIA4)多克隆抗體
Polyclonal Antibody to Protein Disulfide Isomerase A4 (PDIA4)
PDI-A4; ERP70; ERP72; Protein Disulfide Isomerase-Associated 4; Endoplasmic reticulum resident protein 70
- 編號PAD774Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原RPD774Hu01-蛋白二硫化物異構(gòu)酶A4(PDIA4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
Figure. FITC staining on IHC-P; Samples: Human Hela Cells.
DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Pancreas Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human PDIA4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Figure. Western Blot; Sample: Recombinant PDIA4, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: Porcine Liver lysate; Lane3: Porcine Cerebrum lysate; Lane4: A431 cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: PDIA4 knockout Hela cell lysate;
Predicted MW: 73kd
Observed MW: 73kd
Primary Ab: 3μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: NIH/3T3 cell lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human PDIA4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對PDIA4的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別PDIA4。
用法
Western blotting: 0.5-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPD774Hu01 | 蛋白二硫化物異構(gòu)酶A4(PDIA4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD774Hu01 | 蛋白二硫化物異構(gòu)酶A4(PDIA4)多克隆抗體 | WB,IHC |
| MAD774Hu21 | 蛋白二硫化物異構(gòu)酶A4(PDIA4)單克隆抗體 | WB; IHC; ICC; IP. |
| SED774Hu | 蛋白二硫化物異構(gòu)酶A4(PDIA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD774Hu | 蛋白二硫化物異構(gòu)酶A4(PDIA4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Indonesian Journal of Clinical Pathology and Medical Laboratory | Keluarga Disulfit Protein Isomerase Anggota 4(PDIA4) di Kanker Payudara dengan metastasis[Unair.ac: Source] |
| PLoS One | A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4[pubmed:28650993] |
| Acta Diabetol | Circulating protein disulfide isomerase family member 4 is associated with type 2 diabetes mellitus, insulin sensitivity, and obesity[Pubmed:35460376] |