細胞毒性T-淋巴細胞關聯(lián)抗原4(CTLA4)重組抗體
Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)
CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4
- 編號RAB230Hu71
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 來源重組單抗制備
- 宿主CHO
- 效價(克隆號)-
- Ig亞型 IgG
- 純化方式蛋白A + 蛋白G親和純化
- 標記物生物素
- 免疫原-
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應用WB; IF; ICC; IHC; IP; FCM.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載英文說明書 中文說明書
- 規(guī)格20μl100μl200μl1ml10ml
- 價格¥ 1080¥ 2520¥ 3600¥ 9000¥ 36000
- 欲了解實際交易價格和更多情況，請與當?shù)亟涗N商聯(lián)系!
產品包裝(模擬)
產品包裝(模擬)
Briefly, biotin labelled CTLA4 Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to rh CTLA4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of rh CTLA4 and CTLA4 Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.06607 ug/ml.
通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對CTLA4的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別CTLA4。
用法
免疫印跡：0.2-2μg/mL;1:500-5000
免疫組織化學：5-20μg/mL;1:50-200
免疫細胞化學：5-20μg/mL;1:50-200
最佳稀釋倍數(shù)最終由用戶決定。
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產品
參考文獻
| 雜志 | 參考文獻 |
| Int Immunopharmacol | Circulating CTLA-4 levels and CTLA4 polymorphisms associate with disease condition and progression and hepatocellular carcinoma patients' survival in chronic …[Pubmed: 32163858] |